Figure 2
From: Hyperthermophilic endoglucanase for in planta lignocellulose conversion
Western blot (A) after SDS-PAGE and zymography (B) after SDS-PAGE in the presence of 0.15% (w/v) CMC, representing different purification steps following SSO1354 expression in tobacco. Lanes contain 1 μg of purified SSO1354. The recombinant enzyme was detected with an anti-penta-His primary antibody and an AP-conjugated goat anti-mouse secondary antibody. The multiple bands may represent miscellaneous forms of N-glycosylation.