Influence of temperature ( A ) and pH ( B ) on SSO1354 activity. Conversion of Azo-CMC was measured at 60–99°C. The maximum activity measured at 90°C was set at 100%. Influence of pH on SSO1354 activity was measured by Azo-CMC conversion at pH values between 2.0 and 7.0. The maximum activity measured at pH 4.5 was set at 100%.