Figure 6

ADH activities. Strain JDY4 was transformed with plasmids overexpressing ADH1, ADH2, ADH3, ADH4, ADH6 or SFA1. Transformants were grown in selective SCD medium and ADH activity with either 50 mM isobutyraldehyde (A) or 10 mM acetaldehyde (B) as a substrate determined in crude extracts and normalized to the amount of protein.