Figure 7

Isobutanol production with strains expressing the cytosolic valine pathway. Fermentation experiments were performed aerobically at 30°C in shake flasks in selective SCD-val medium containing 40 g/L glucose. Transformants were pregrown aerobically in fermentation medium, harvested and inoculated in fresh medium. Experiments were performed in triplicate with given standard deviations. Panel A: isobutanol production of Isoy17 expressing different plasmid combinations encoding for: empty vectors (closed circle); Aro10 and Adh2 (closed square); wild-type Ilv2, Ilv5 and Ilv3 (closed triangle), wild-type Ilv2, Ilv5, Ilv3, Aro10 and Adh2 (inverted closed triangle); truncated Ilv2ΔN54, Ilv5ΔN48 and Ilv3ΔN19 (closed diamond); truncated Ilv2ΔN54, Ilv5ΔN48, Ilv3ΔN19, Aro10 and Adh2 (open circle), codon-optimized truncated Ilv2ΔN54, Ilv5ΔN48 and Ilv3ΔN19 (open square), codon-optimized truncated Ilv2ΔN54, Ilv5ΔN48, Ilv3ΔN19, Aro10 and Adh2 (open triangle). Panel B: isobutanol yield of Isoy17 expressing different plasmid combinations encoding for genes which are indicated below the axis. Plus symbols denote overexpression of the indicated gene.