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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production

Figure 2

Gas chromatography-mass spectrometry analysis of fatty acid ethyl esters in lipid extracts from strains CB0 and CB2. Cells were cultured in SD medium lacking uracil containing 2% glucose. Neutral lipids were purified from total lipid extracts of lyophilized cells by preparative TLC and subjected to GC-MS analyses. The corresponding mass spectra of FAEEs are shown in Additional file 6. (A) Gas chromatograms of FAEE standards: 1. lauric acid ethyl ester [12:0]; 2. myristic acid ethyl ester [14:0]; 3. palmitic acid ethyl ester [16:0]; 4. palmitoleic acid ethyl ester [16:1]; 5. heptadecanoic acid ethyl ester [17:0]; 6. octadecanoic acid ethyl ester [18:0]; 7. oleic acid ethyl ester [18:1]. (B) Gas chromatogram of FAEEs from lipid extract of CB0 containing 25 μg internal standard, heptadecanoic acid ethyl ester [17:0]. (C) Gas chromatogram of FAEEs from lipid extract of CB2 containing 25 μg internal standard, heptadecanoic acid ethyl ester [17:0].

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