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Table 3 List of strains used in this study and their genotypes

From: Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production

Strain

Genotype

Plasmid

Source

E. coli strains

   

E. coli DH5α

supE 44 lacU 169 (ϕ80lacZ ΔM15) hsdR 17 recA 1 endA 1 gyrA 96 thi-1 relA 1

  

S. cerevisiae strains

   

CEN.PK 113-5D

MAT a MAL2-8 c SUC2 ura3-52

 

P. Kötter, University of Frankfurt, Germany

CB0

CEN.PK 113-5D

pSP-GM2

This study

CB1

CEN.PK 113-5D

pSP-GM2 carrying WS gene from A. baylyi ADP1

This study

CB2

CEN.PK 113-5D

pSP-GM2 carrying WS gene from M. hydrocarbonoclasticus DSM 8798

This study

CB3

CEN.PK 113-5D

pSP-GM2 carrying WS gene from R. opacus PD630

This study

CB4

CEN.PK 113-5D

pSP-GM2 carrying WS gene from Mus musculus C57BL/6

This study

CB5

CEN.PK 113-5D

pSP-GM2 carrying WS gene from P. arcticus 273-4

This study

CB2A

CEN.PK 113-5D

pSP-GM2 carrying WS gene from M. hydrocarbonoclasticus DSM 8798 and ACC1

This study

  1. WS: wax ester synthase