Table 1 Metabolite concentrations [mean (sem)] and fold change of down-regulated COMT versus wild-type (WT) switchgrass
Metabolite | COMT concentration (μg/ml) | WT concentration (μg/ml) | COMT/WT fold change | P-value |
|---|---|---|---|---|
iso-sinapyl alcohol | 0.83 (0.06) | nd | ∞ | 0.000 |
iso-sinapic acid | 0.11 (0.01) | nd | ∞ | 0.000 |
15.09 354 239 620 lignan a | 0.34 (0.02) | nd | ∞ | 0.000 |
15.18 354 219 M + 530 (iso-sinapyl-phenolic) | 0.11 (0.01) | nd | ∞ | 0.000 |
5-hydroxyconiferyl alcohol-4-O-glucoside | 0.08 (0.01) | 0.00 (0.00) | 76.05 | 0.000 |
5-hydroxyconiferyl alcohol-5-O-glucoside | 0.17 (0.01) | 0.00 (0.00) | 59.83 | 0.000 |
iso-syringin | 2.60 (0.32) | 0.06 (0.01) | 42.79 | 0.000 |
12.37 428 327 209 413 | 0.39 (0.02) | 0.03 (0.01) | 14.09 | 0.000 |
3,4-dihydroxybenzoic acid | 7.52 (3.72) | 1.19 (0.34) | 6.34 | 0.096 |
xanthine | 1.14 (0.14) | 0.43 (0.18) | 2.68 | 0.019 |
hypoxanthine | 2.85 (0.96) | 1.07 (0.17) | 2.65 | 0.080 |
succinic acid | 14.98 (3.97) | 5.78 (1.04) | 2.59 | 0.041 |
guanosine | 5.18 (0.76) | 2.17 (0.62) | 2.39 | 0.018 |
uracil | 2.02 (0.29) | 0.98 (0.22) | 2.07 | 0.023 |
citraconic acid | 2.25 (0.18) | 1.23 (0.31) | 1.82 | 0.033 |
guanine | 7.24 (0.28) | 4.10 (0.85) | 1.77 | 0.016 |
5-hydroxyferulic acid | 0.46 (0.01) | 0.27 (0.02) | 1.69 | 0.000 |
uridine | 11.86 (0.49) | 7.27 (1.39) | 1.63 | 0.026 |
maleic acid | 65.92 (1.63) | 41.74 (7.23) | 1.58 | 0.023 |
vanillin | 26.20 (1.63) | 16.81 (3.48) | 1.56 | 0.060 |
secoisolariciresinol | 2.27 (0.13) | 1.47 (0.38) | 1.54 | ns |
5-oxo-proline | 116.72 (6.82) | 77.40 (7.46) | 1.51 | 0.007 |
adenine | 10.19 (0.72) | 7.25 (0.91) | 1.41 | 0.045 |
1-O-trans- feruloylglycerol | 1.05 (0.05) | 0.77 (0.11) | 1.37 | 0.065 |
ferulic acid | 7.06 (0.17) | 5.50 (0.14) | 1.28 | 0.000 |
5-hydroxyconiferaldehyde (13.08 M + 338 323 ) | 0.26 (0.02) | 0.20 (0.01) | 1.28 | 0.029 |
adenosine | 9.62 (0.66) | 7.58 (0.86) | 1.27 | ns |
p-coumaric acid | 21.80 (0.21) | 19.31 (0.57) | 1.13 | 0.08 |
caffeic acid | 0.58 (0.04) | 0.53 (0.04) | 1.11 | ns |
p-hydroxybenzaldehyde | 4.63 (0.33) | 4.24 (0.80) | 1.09 | ns |
coniferyl alcohol | 3.34 (0.10) | 3.65 (0.22) | 0.92 | ns |
5-hydroxyconiferyl alcohol | 1.22 (0.06) | 1.36 (0.08) | 0.90 | ns |
coniferyl aldehyde | 0.35 (0.01) | 0.45 (0.07) | 0.78 | ns |
guaiacylglycerol | 5.25 (0.23) | 6.79 (0.53) | 0.77 | 0.047 |
sinapyl aldehyde | 0.17 (0.02) | 0.22 (0.03) | 0.77 | ns |
syringin | 0.23 (0.01) | 0.30 (0.03) | 0.77 | 0.093 |
sinapyl alcohol | 2.51 (0.06) | 3.48 (0.20) | 0.72 | 0.004 |
syringylglycerol | 2.99 (0.09) | 4.48 (0.25) | 0.67 | 0.001 |
p-hydroxyphenylpyruvic acid | 0.63 (0.05) | 0.96 (0.09) | 0.65 | 0.021 |
guaiacylglycerol glycoside (14.51 297 608 593) | 3.53 (0.17) | 5.57 (0.69) | 0.63 | 0.038 |
syringaresinol | 0.07 (0.00) | 0.14 (0.01) | 0.52 | 0.001 |
guaiacylglycerol glycoside (14.57 297 608 593) | 3.59 (0.19) | 5.98 (0.88) | 0.60 | 0.050 |
syringylglycerol glycoside (16.12 327 361 239) | 3.91 (0.22) | 7.88 (1.34) | 0.50 | 0.035 |
pinoresinol | 0.22 (0.03) | 0.45 (0.07) | 0.49 | 0.031 |
guaiacylglycerol glycoside (15.87 297 361 323) | 7.42 (0.30) | 17.96 (4.41) | 0.41 | 0.073 |
hydroxymethylfurfural | 0.72 (0.12) | 3.40 (1.41) | 0.21 | ns |