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Table 1 Bacterial strains and plasmids used in this study

From: Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase

Plasmid or strain Relevant genotype Reference or source
Plasmids   
pET-28a(+) pBR322 ori lacI T7lac Kanr Novagen
pACYCDuet-1 P15A ori lacI T7lac Cmr Novagen
pCOLADuet-1 ColA ori lacI T7lac Kanr Novagen
pBAD18 pBR322 ori ara BAD Ampr 37
pBAD30 pACYC184 ori ara BAD Ampr 37
pBAD33 pACYC184 ori ara BAD Cmr 37
pLL8 pCOLADuet-1 harboring ‘AcTesA from A. baylyi This study
pLL18 pACYCDuet-1 harboring ‘AcTesA from A. baylyi This study
pLL28 pET-28a(+) harboring ‘AcTesA from A. baylyi This study
pLL38 pBAD18 harboring ‘AcTesA from A. baylyi This study
pLL48 pBAD30 harboring ‘AcTesA from A. baylyi This study
pLL58 pBAD33 harboring ‘AcTesA from A. baylyi This study
pS10A pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Ser10 residue This study
pG48A pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Gly48 residue This study
pN77A pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Asn77 residue This study
pD158A pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Asp158 residue This study
pH161A pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at His161 residue This study
Strains   
BL21 (DE3) F-ompT gal dcm lon hsdSB(r B -m B -) λ(DE3) Invitrogen
LL8 E. coli BL21 (DE3) bearing pLL8 This study
LL18 E. coli BL21 (DE3) bearing pLL18 This study
LL28 E. coli BL21 (DE3) bearing pLL28 This study
LL38 E. coli BL21 (DE3) bearing pLL38 This study
LL48 E. coli BL21 (DE3) bearing pLL48 This study
LL58 E. coli BL21 (DE3) bearing pLL58 This study
S10A E. coli BL21 (DE3) bearing pS10A This study
G48A E. coli BL21 (DE3) bearing pG48A This study
N77A E. coli BL21 (DE3) bearing pN77A This study
D158A E. coli BL21 (DE3) bearing pD158A This study
H161A E. coli BL21 (DE3) bearing pH161A This study