Table 1 Bacterial strains and plasmids used in this study

Plasmids

pET-28a(+)

pBR322 ori lacI T7lac Kan^r

Novagen

pACYCDuet-1

P15A ori lacI T7lac Cm^r

Novagen

pCOLADuet-1

ColA ori lacI T7lac Kan^r

Novagen

pBAD18

pBR322 ori ara BAD Amp^r

37

pBAD30

pACYC184 ori ara BAD Amp^r

37

pBAD33

pACYC184 ori ara BAD Cm^r

37

pLL8

pCOLADuet-1 harboring ‘AcTesA from A. baylyi

This study

pLL18

pACYCDuet-1 harboring ‘AcTesA from A. baylyi

This study

pLL28

pET-28a(+) harboring ‘AcTesA from A. baylyi

This study

pLL38

pBAD18 harboring ‘AcTesA from A. baylyi

This study

pLL48

pBAD30 harboring ‘AcTesA from A. baylyi

This study

pLL58

pBAD33 harboring ‘AcTesA from A. baylyi

This study

pS10A

pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Ser¹⁰ residue

This study

pG48A

pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Gly⁴⁸ residue

This study

pN77A

pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Asn⁷⁷ residue

This study

pD158A

pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at Asp¹⁵⁸ residue

This study

pH161A

pCOLADuet-1 harboring ‘AcTesA with a mutation to Ala at His¹⁶¹ residue

This study

Strains

BL21 (DE3)

F^-ompT gal dcm lon hsdSB(r _B ^-m _B ^-) λ(DE3)

Invitrogen

LL8

E. coli BL21 (DE3) bearing pLL8

This study

LL18

E. coli BL21 (DE3) bearing pLL18

This study

LL28

E. coli BL21 (DE3) bearing pLL28

This study

LL38

E. coli BL21 (DE3) bearing pLL38

This study

LL48

E. coli BL21 (DE3) bearing pLL48

This study

LL58

E. coli BL21 (DE3) bearing pLL58

This study

S10A

E. coli BL21 (DE3) bearing pS10A

This study

G48A

E. coli BL21 (DE3) bearing pG48A

This study

N77A

E. coli BL21 (DE3) bearing pN77A

This study

D158A

E. coli BL21 (DE3) bearing pD158A

This study

H161A

E. coli BL21 (DE3) bearing pH161A

This study