Table 2 Primers used in this study
Name | Sequence (5’→3’) |
|---|---|
AcTesA-NcoF | CTAGCCATGG GCAAAACCATTCTTATCTTAGGCG |
AcTesA-BamHR | CAGGGATCC TTATAAAGCGCCTTTAATATATGG |
rAcTesA-SacF | GACGAGCTC AGGAGGTAAAAAAACATGGGCAAAACCATTCTTATCTTAGGCG |
AcTesA-HindR | CTACCAAGCTT TTATAAAGCGCCTTTAATATATGG |
S10A-F | CTTATCTTAGGCGACGCT CTGAGTGCGGGTTATGG |
S10A-R | CTCAGAGC GTCGCCTAAGATAAGAATGGTTTTGCC |
G48A-F | GCCAGTGTAAGTG C G GAAACCACCAGTGGTGC |
G48A-R | GTTTCCGC ACTTACACTGGCATTAATGACTTTATG |
N77A-F | GAGCTTGGTGGTGCT GATGCATTAAGAGGAC |
N77A-R | GCATCAGC ACCACCAAGCTCAATGACCACCAC |
D158A-F | CTAATGCAAAATGCC CAGATCCATCCAAATGC |
D158A-R | CTGGGC ATTTTGCATTAGACTTTTGTGTCCAG |
H161A-F | CAAAATGACCAGATCGCT CCAAATGCCAAAGCCCAG |
H161A-R | CATTTGGAGC GATCTGGTCATTTTGCATTAGACTTTTG |