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Table 1 Contribution of background activities of CDH IIA and ascorbic acid, respectively, to the total activity measured with various concentrations of PMO (PMO-02916)

From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Assay PMO concentration H2O2production PMO activity Background activity
[mg mL-1] [μmol L-1min-1] [μmol L-1min-1] [%]
Ascorbate 2.87 5.39 ± 0.23 4.90 9.1
  1.43 3.61 ± 0.22 3.12 13
  0.72 2.34 ± 0.05 1.85 21
  0.18 1.05 ± 0.01 0.56 47
  0.02 0.63 ± 0.02 0.14 78
Blank 0 0.49 ± 0.02 0 0
CDH IIA 5.74 6.21 ± 0.22 5.51 11
  1.43 2.57 ± 0.11 1.87 27
  0.72 1.77 ± 0.11 1.07 40
  0.18 0.94 ± 0.05 0.24 74
  0.02 0.74 ± 0.05 0.04 94
Blank 0 0.70 ± 0.04 0 0