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Table 1 Contribution of background activities of CDH IIA and ascorbic acid, respectively, to the total activity measured with various concentrations of PMO (PMO-02916)

From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Assay

PMO concentration

H2O2production

PMO activity

Background activity

[mg mL-1]

[μmol L-1min-1]

[μmol L-1min-1]

[%]

Ascorbate

2.87

5.39 ± 0.23

4.90

9.1

 

1.43

3.61 ± 0.22

3.12

13

 

0.72

2.34 ± 0.05

1.85

21

 

0.18

1.05 ± 0.01

0.56

47

 

0.02

0.63 ± 0.02

0.14

78

Blank

0

0.49 ± 0.02

0

0

CDH IIA

5.74

6.21 ± 0.22

5.51

11

 

1.43

2.57 ± 0.11

1.87

27

 

0.72

1.77 ± 0.11

1.07

40

 

0.18

0.94 ± 0.05

0.24

74

 

0.02

0.74 ± 0.05

0.04

94

Blank

0

0.70 ± 0.04

0

0