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Table 2 Fermentation of PMO enzymes

From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Enzyme

Harvest time

Wet biomass

Protein conc.

Vol. act.

Spec. act.

TTP conc.a

[h]

[g L-1]

[g L-1]

[U L-1]

[U g-1]

[g L-1]

PMO-01867

116.5

388

1.57

0.46

0.38

0.31

PMO-02916

145.0

447

2.76

4.46

1.59

0.79

PMO-03328

115.5

391

1.82

0.72

0.39

0.05

PMO-08760

119.5

441

1.33

1.46

1.05

0.34

  1. a Total Target Protein concentration was calculated from the total protein concentration using the specific activity of the purified PMO.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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