Purification step | Total proteina[g] | Total activity [U] | Specific Activity [U g-1] | Purification [−fold] | Yield [%] |
---|---|---|---|---|---|
PMO-01867 | |||||
Culture supernatant (2.5 L) | 3.935 | 1.48 | 0.38 | 1 | 100 |
Phenyl Sepharose | 0.536 | 0.39 | 0.74 | 2.0 | 27 |
Q-Source | 0.211 | 0.25 | 1.18 | 3.2 | 17 |
Gel filtration | 0.060 | 0.12 | 1.91 | 5.1 | 7.8 |
PMO-02916 | |||||
Culture supernatant (2.1 L) | 5.800 | 9.21 | 1.59 | 1 | 100 |
Phenyl Sepharose | 0.334 | 1.10 | 3.28 | 2.1 | 12 |
Q-Source | 0.140 | 0.78 | 5.57 | 3.5 | 8 |
PMO-03328 | |||||
Culture supernatant (2.9 L) | 5.275 | 2.08 | 0.39 | 1 | 100 |
Phenyl Sepharose | 0.235 | 1.57 | 6.70 | 17.0 | 76 |
Q-Source | 0.074 | 0.70 | 9.52 | 24.1 | 34 |
Gel filtration | 0.007 | 0.11 | 15.76 | 39.9 | 5.4 |
PMO-08760 | |||||
Culture supernatant (2.3 L) | 3.052 | 3.19 | 1.05 | 1 | 100 |
Phenyl Sepharose | 0.158 | 0.30 | 1.87 | 1.8 | 9 |
Q-Source | 0.047 | 0.12 | 2.54 | 2.4 | 4 |
Gel filtration | 0.005 | 0.02 | 4.08 | 3.9 | 0.6 |