Skip to main content

Table 3 Purification scheme of recombinant PMO enzymes

From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Purification step Total proteina[g] Total activity [U] Specific Activity [U g-1] Purification [−fold] Yield [%]
PMO-01867      
Culture supernatant (2.5 L) 3.935 1.48 0.38 1 100
Phenyl Sepharose 0.536 0.39 0.74 2.0 27
Q-Source 0.211 0.25 1.18 3.2 17
Gel filtration 0.060 0.12 1.91 5.1 7.8
PMO-02916      
Culture supernatant (2.1 L) 5.800 9.21 1.59 1 100
Phenyl Sepharose 0.334 1.10 3.28 2.1 12
Q-Source 0.140 0.78 5.57 3.5 8
PMO-03328      
Culture supernatant (2.9 L) 5.275 2.08 0.39 1 100
Phenyl Sepharose 0.235 1.57 6.70 17.0 76
Q-Source 0.074 0.70 9.52 24.1 34
Gel filtration 0.007 0.11 15.76 39.9 5.4
PMO-08760      
Culture supernatant (2.3 L) 3.052 3.19 1.05 1 100
Phenyl Sepharose 0.158 0.30 1.87 1.8 9
Q-Source 0.047 0.12 2.54 2.4 4
Gel filtration 0.005 0.02 4.08 3.9 0.6
  1. aAll protein concentrations were measured with the Bradford assay.