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Table 3 Purification scheme of recombinant PMO enzymes

From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay

Purification step

Total proteina[g]

Total activity [U]

Specific Activity [U g-1]

Purification [−fold]

Yield [%]

PMO-01867

     

Culture supernatant (2.5 L)

3.935

1.48

0.38

1

100

Phenyl Sepharose

0.536

0.39

0.74

2.0

27

Q-Source

0.211

0.25

1.18

3.2

17

Gel filtration

0.060

0.12

1.91

5.1

7.8

PMO-02916

     

Culture supernatant (2.1 L)

5.800

9.21

1.59

1

100

Phenyl Sepharose

0.334

1.10

3.28

2.1

12

Q-Source

0.140

0.78

5.57

3.5

8

PMO-03328

     

Culture supernatant (2.9 L)

5.275

2.08

0.39

1

100

Phenyl Sepharose

0.235

1.57

6.70

17.0

76

Q-Source

0.074

0.70

9.52

24.1

34

Gel filtration

0.007

0.11

15.76

39.9

5.4

PMO-08760

     

Culture supernatant (2.3 L)

3.052

3.19

1.05

1

100

Phenyl Sepharose

0.158

0.30

1.87

1.8

9

Q-Source

0.047

0.12

2.54

2.4

4

Gel filtration

0.005

0.02

4.08

3.9

0.6

  1. aAll protein concentrations were measured with the Bradford assay.