Table 4 Molecular masses and glycosylation of PMO enzymes
Enzyme | SDS-PAGE | Calculated | Glycan mass | Glycan mass | Putative glycosyl. sites | |
|---|---|---|---|---|---|---|
[kDa] | [kDa] | [kDa] | [%] | O-Glyc. | N- Glyc. | |
PMO-01867 | 39.6 ± 0.7 | 32.7 | 6.9 | 17.5 | 5 | 0 |
PMO-02916 | 57.5 ± 0.8 | 34.3 | 23.2 | 40.4 | 27 | 2 |
PMO-03328 | 23.7 ± 0.6 | 23.2 | 0.0 | 0.0 | 5 | 0 |
PMO-08760 | 41.4 ± 0.4 | 32.2 | 9.2 | 22.2 | 16 | 0 |