Detection of transgene presence and expression. (A) Agarose gels showing the presence of the transgene by PCR. Leaves from 6-week old plants were used. (B) RT-PCR analysis confirming expression of IRX7 and IRX9 genes respectively in stems of transformed plants. Actin2 was amplified as control. cDNA was prepared from stems (top 5 cm) of 6-week old plants.