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Table 1 Plant lines and primers used for genotyping

From: Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants

Mutant Line Gene Locus Insertion line Primer sequence
irx7 At2g28110 SALK_120296 5’-CGTCATTGATGAGTGATCGTG-3’ fwd
5’- TGCAAAATTTGTCATTGTCAC-3’ rev
irx8-6 At5g54690 SALK_008642 5’-TCAAGAAAAATCATCAACCGG-3’fwd
5’-AACAGCATTAGACCCGAAACC-3’ rev
irx9-2 At2g37090 SALK_057033 5’- GCTGGTAAGGCCTCATTTTTC-3’ fwd
5’- AACTTACCAACCCACCCATTC-3’ rev
  1. The primers were used to identify transformants that were homozygous for the T-DNA insertions yielding the irx9-2, irx7 and irx8 mutants. Primer sets of right- and left-border primers (RP+LP) specific for each T-DNA insertion were generated from the SIGnAL T-DNA Primer Design website (http://signal.salk.edu/tdnaprimers.2.html). T-DNA left border primers used were LBa1 (5’-TGGTTCACGTAGTGGGCCATCG-3’) and LBb1.3 (5’-ATTTTGCCGATTTCGGAAC-3’).