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Table 2 Primers used to confirm presence and expression of the transgenes

From: Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants

Primer name Primer sequence
pVND6_irx7/9_FW 5’-GAATCCACCATCATCGGAGT-3’
pVND6_irx7_RV 5’-TCGGCTTCATACGGATCTTC-3’
pVND6_irx9_RV 5’-CCACAATAAGGGAGGTGGAA-3’
pVND7_irx7/9_FW 5’-ATGCACGAACCAGATGATGA-3’
pVND7_irx7_RV 5’-CGAGAAGCGAAAAGCTTAGG-3’
pVND7_irx9_RV 5’-TCAAAAGGTTGGGGAGGAAT-3’
IRX7_RT-PCR_FW 5’-ATGACAACACATAAACATAGAAGAAC-3’
IRX7_RT-PCR_RV 5’-CAAGAAAGAGTTTGACCTTCTAACA-3’
IRX9_RT-PCR_FW 5’-ATGGGATCTCTAGAGAGATCA-3’
IRX9_RT-PCR_RV 5’-GGTGCTTAAACGTGTTCTTGTG-3’
ACT2_FW 5’-CTCAAA GACCAGCTCTTCCATC-3’
ACT2_RV 5’-GCCTTTGATCTTGAGAGCTTAG-3’.