Table 2 Primers used to confirm presence and expression of the transgenes
Primer name | Primer sequence |
|---|---|
pVND6_irx7/9_FW | 5’-GAATCCACCATCATCGGAGT-3’ |
pVND6_irx7_RV | 5’-TCGGCTTCATACGGATCTTC-3’ |
pVND6_irx9_RV | 5’-CCACAATAAGGGAGGTGGAA-3’ |
pVND7_irx7/9_FW | 5’-ATGCACGAACCAGATGATGA-3’ |
pVND7_irx7_RV | 5’-CGAGAAGCGAAAAGCTTAGG-3’ |
pVND7_irx9_RV | 5’-TCAAAAGGTTGGGGAGGAAT-3’ |
IRX7_RT-PCR_FW | 5’-ATGACAACACATAAACATAGAAGAAC-3’ |
IRX7_RT-PCR_RV | 5’-CAAGAAAGAGTTTGACCTTCTAACA-3’ |
IRX9_RT-PCR_FW | 5’-ATGGGATCTCTAGAGAGATCA-3’ |
IRX9_RT-PCR_RV | 5’-GGTGCTTAAACGTGTTCTTGTG-3’ |
ACT2_FW | 5’-CTCAAA GACCAGCTCTTCCATC-3’ |
ACT2_RV | 5’-GCCTTTGATCTTGAGAGCTTAG-3’. |