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Table 2 Top 50 expressed genes based on normalized expression level (RPKM values)

From: RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803

Gene ID

Control-24h

Control-48h

Control-72h

Ethanol-24h-r1

Ethanol-24h-r2

Ethanol-48h-r1

Ethanol-48h-r2

Ethanol-72h-r1

Ethanol-72h-r2

Discription

Cellular role

sll1744

7.42E+05

8.96E+05

9.57E+05

6.14E+05

6.66E+05

6.65E+05

3.97E+05

7.24E+05

6.49E+05

50S ribosomal protein L1

Protein synthesis

sll1745

3.42E+06

3.74E+06

3.97E+06

2.80E+06

3.01E+06

2.74E+06

1.82E+06

2.90E+06

2.54E+06

50S ribosomal protein L10

Protein synthesis

sll1743

3.56E+05

5.73E+05

6.30E+05

3.00E+05

3.86E+05

4.17E+05

2.23E+05

4.90E+05

4.11E+05

50S ribosomal protein L11

Protein synthesis

sll1746

6.76E+05

5.38E+05

7.26E+05

1.66E+05

2.49E+05

2.90E+05

1.78E+05

2.78E+05

2.58E+05

50S ribosomal protein L12

Protein synthesis

sll0767

2.07E+06

9.55E+05

2.40E+06

9.92E+05

9.52E+05

7.76E+05

6.25E+05

7.37E+05

6.30E+05

50S ribosomal protein L20

Protein synthesis

sll1801

3.47E+05

5.18E+05

7.09E+05

1.62E+05

2.61E+05

4.74E+05

2.32E+05

5.43E+05

5.32E+05

50S ribosomal protein L23

Protein synthesis

sll1807

4.12E+05

6.41E+05

9.77E+05

1.72E+05

3.12E+05

5.68E+05

2.51E+05

6.14E+05

5.94E+05

50S ribosomal protein L24

Protein synthesis

ssl1426

5.89E+06

2.35E+06

5.49E+06

4.31E+06

4.18E+06

2.74E+06

2.30E+06

2.22E+06

2.17E+06

50S ribosomal protein L35

Protein synthesis

slr2067

1.00E+06

2.39E+06

4.47E+06

3.52E+05

1.16E+06

2.61E+06

8.80E+05

3.28E+06

3.45E+06

Allophycocyanin alpha subunit

Energy metabolism

slr1986

8.68E+05

1.50E+06

2.98E+06

2.53E+05

6.79E+05

1.35E+06

4.90E+05

1.72E+06

1.75E+06

Allophycocyanin beta subunit

Energy metabolism

slr1198

1.72E+06

1.22E+06

1.77E+06

1.08E+06

1.33E+06

1.10E+06

7.41E+05

1.08E+06

1.12E+06

Ant ioxidant protein

Unclassified

sll1322

2.11E+05

6.36E+05

8.15E+05

1.31E+05

4.50E+05

6.64E+05

3.23E+05

9.71E+05

7.65E+05

ATP synthase A chain of CF(0)

Energy metabolism

ssl2615

4.95E+05

1.22E+06

2.06E+06

2.69E+05

7.74E+05

1.33E+06

5.88E+05

1.82E+06

1.57E+06

ATP synthase C chain of CF(0)

Energy metabolism

sll1323

2.28E+05

5.71E+05

8.68E+05

1.17E+05

3.22E+05

6.10E+05

2.82E+05

8.06E+05

6.33E+05

ATP synthase subunit b' of CF(0)

Energy metabolism

sll1099

4.39E+05

9.53E+05

1.51E+06

1.94E+05

4.07E+05

7.88E+05

3.36E+05

9.11E+05

8.63E+05

Elongation factor Tu

Protein synthesis

ssl0020

2.52E+06

6.33E+05

7.00E+05

6.84E+05

7.65E+05

8.17E+05

7.10E+05

8.75E+05

7.41E+05

Ferredoxin I, essential for growth

Energy metabolism

sll0018

1.97E+05

7.10E+05

1.21E+06

1.36E+05

2.37E+05

7.34E+05

3.61E+05

1.04E+06

1.08E+06

Fructose-bisphosphate aldolase, class II

Unclassified

ssl0483

1.48E+06

5.07E+05

6.64E+05

6.22E+05

7.23E+05

6.12E+05

5.26E+05

6.88E+05

6.09E+05

Hypothetical protein

No Data

slr0144

8.77E+05

5.27E+05

5.68E+05

3.89E+05

4.55E+05

3.04E+05

2.59E+05

2.49E+05

2.15E+05

Hypothetical protein

Hypothetical proteins

slr1470

6.22E+05

6.04E+05

8.56E+05

4.92E+05

6.35E+05

6.70E+05

4.85E+05

7.30E+05

6.91E+05

Hypothetical protein

Hypothetical proteins

slr0373

4.60E+05

5.51E+05

1.65E+06

1.51E+05

3.63E+05

7.25E+05

2.74E+05

9.89E+05

8.96E+05

Hypothetical protein

No Data

slr0749

9.08E+05

6.46E+05

1.19E+06

7.58E+05

1.34E+06

7.50E+05

4.19E+05

4.46E+05

4.20E+05

Light-independent protochlorophyllide reductase ironprotein subunit ChlL

Cofactor biosynthesis

slr0749

9.08E+05

6.46E+05

1.19E+06

7.58E+05

1.34E+06

7.50E+05

4.19E+05

4.46E+05

4.20E+05

Light-independent protochlorophyllide reductase iron protein subunit ChlL

Energy metabolism

sll1342

3.21E+05

5.41E+05

7.43E+05

2.32E+05

3.29E+05

4.17E+05

2.38E+05

4.80E+05

5.42E+05

NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Energy metabolism

slr1834

1.46E+06

1.55E+07

3.92E+07

1.23E+06

5.28E+06

1.28E+07

3.37E+06

1.50E+07

1.66E+07

P700 apoprotein subunit Ia

Energy metabolism

slr1835

1.21E+06

5.21E+06

1.16E+07

6.51E+05

2.62E+06

5.15E+06

1.74E+06

5.93E+06

5.79E+06

P700 apoprotein subunit Ib

Energy metabolism

sll0819

1.20E+06

6.37E+05

6.10E+05

3.32E+05

2.99E+05

3.72E+05

2.59E+05

4.10E+05

3.91E+05

Photosystem I reaction center subunit III precursor

Energy metabolism

slr0737

1.85E+06

1.15E+06

2.11E+06

9.16E+05

1.10E+06

1.20E+06

8.97E+05

1.34E+06

1.38E+06

Photosystem I subunit II

Energy metabolism

smr0004

4.02E+06

3.59E+06

5.24E+06

1.43E+06

1.45E+06

1.78E+06

1.44E+06

1.60E+06

1.58E+06

Photosystem I subunit VIII

Energy metabolism

slr1655

1.08E+07

5.24E+06

5.95E+06

8.83E+06

8.55E+06

7.79E+06

5.87E+06

7.50E+06

6.75E+06

Photosystem I subunit XI

Energy metabolism

slr0906

2.96E+05

5.56E+05

4.91E+05

1.78E+05

4.12E+05

5.30E+05

2.60E+05

7.12E+05

6.19E+05

Photosystem II core light harvest ing protein

Energy metabolism

sll0851

2.98E+05

1.21E+06

2.33E+06

1.71E+05

4.95E+05

1.05E+06

3.40E+05

9.30E+05

9.78E+05

Photosystem II CP43 protein

Energy metabolism

slr1311

2.37E+06

1.75E+06

2.79E+06

8.49E+05

1.83E+06

2.41E+06

1.39E+06

2.73E+06

2.44E+06

Photosystem II D1 protein

Energy metabolism

sll1867

1.22E+06

1.06E+06

1.62E+06

5.25E+05

1.01E+06

1.33E+06

7.68E+05

1.54E+06

1.59E+06

Photosystem II D1 protein

Energy metabolism

sll0849

1.31E+05

1.09E+06

1.59E+06

9.21E+04

3.93E+05

1.05E+06

3.10E+05

1.21E+06

1.20E+06

Photosystem II react ion center D2 protein

Energy metabolism

slr0335

4.12E+05

1.38E+06

2.12E+06

2.41E+05

6.30E+05

1.09E+06

5.13E+05

1.30E+06

1.35E+06

Phycobilisome core-membrane linker polypept ide

Energy metabolism

sll1580

3.09E+06

2.89E+06

2.91E+06

1.93E+06

2.24E+06

2.18E+06

1.69E+06

2.23E+06

1.79E+06

Phycobilisome rod linker polypept ide

Energy metabolism

sll1579

2.30E+06

2.95E+06

3.31E+06

1.33E+06

2.07E+06

2.16E+06

1.46E+06

2.54E+06

2.09E+06

Phycobilisome rod linker polypept ide

Unclassified

slr2051

1.44E+06

6.37E+05

7.37E+05

4.63E+05

5.46E+05

6.26E+05

5.26E+05

6.77E+05

6.51E+05

Phycobilisome rod-core linker polypeptide

Energy metabolism

ssl3093

2.10E+06

1.29E+06

1.36E+06

7.71E+05

9.40E+05

8.59E+05

7.79E+05

1.04E+06

7.32E+05

Phycobilisome small rod linker polypeptide

Unclassified

sll1578

2.59E+07

5.16E+07

8.62E+07

1.43E+07

2.65E+07

4.10E+07

2.17E+07

5.20E+07

5.40E+07

Phycocyanin alpha subunit

Energy metabolism

sll1577

2.49E+07

5.74E+07

8.88E+07

1.88E+07

3.58E+07

5.52E+07

2.70E+07

7.35E+07

7.72E+07

Phycocyanin beta subunit

Energy metabolism

sll1694

5.95E+06

8.44E+05

1.14E+06

2.01E+06

2.12E+06

9.58E+05

1.35E+06

1.07E+06

5.81E+05

Pilin polypept ide PilA1

Cell envelope

sll0199

8.92E+05

6.80E+05

9.96E+05

2.02E+05

5.59E+05

9.24E+05

4.93E+05

8.49E+05

7.72E+05

Plastocyanin

Unclassified

slr0011

1.94E+06

3.06E+06

6.38E+06

1.11E+06

1.85E+06

2.48E+06

1.33E+06

2.73E+06

2.32E+06

Possible Rubisco chaperonin

Hypothetical proteins

slr1841

1.24E+06

2.09E+06

3.27E+06

5.53E+05

1.31E+06

1.70E+06

7.51E+05

1.67E+06

1.89E+06

Probable porin; major outer membrane protein

Unclassified

slr0009

4.48E+05

1.44E+06

2.53E+06

2.81E+05

7.69E+05

1.33E+06

5.61E+05

1.70E+06

1.55E+06

Ribulose bisphosphate carboxylase large subunit

Energy metabolism

slr0012

5.54E+05

8.40E+05

2.10E+06

1.58E+05

2.52E+05

4.06E+05

2.72E+05

4.43E+05

3.79E+05

Ribulose bisphosphate carboxylase small subunit

Energy metabolism

sll1338

9.93E+05

2.56E+06

4.45E+06

5.64E+05

1.23E+06

1.57E+06

9.14E+05

1.79E+06

1.52E+06

Unknown protein

No Data

sll1951

7.54E+05

8.37E+05

1.25E+06

3.14E+05

6.19E+05

7.53E+05

3.27E+05

7.44E+05

8.16E+05

Unknown protein

Unclassified