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Table 3 Genes induced by ethanol exposure *

From: RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803

Gene ID

Description

Ratio-Ethanol-24h-r1 vs. Control-24h

Ratio-Ethanol-24h-r2 vs. Control-24h

Ratio-Ethanol-48h-r1 vs. Control-48h

Ethanol-48h-r2 vs. Control-48h

Ethanol-72h-r1 vs. Control-72h

Ratio-Ethanol-72h-r1 vs. Control-72h

sll0034

putative carboxypeptidase

  

2.69

1.50

  

sll0250

pantothenate metabolism flavoprotein

    

3.00

1.91

sll0289

septum site-determining protein MinD

    

4.56

3.89

sll0300

riboflavin synthase alpha chain

    

4.33

3.33

sll0330

sepiapterine reductase

    

7.09

29.09

sll0368

uracil phosphoribosyltransferase

    

2.33

2.00

sll0374

urea transport system ATP-binding protein

  

2.80

3.60

  

sll0384

Cations and iron carrying protein

  

2.31

1.70

  

sll0450

cytochrome b subunit of nitric oxide reductase

1.62

4.26

    

sll0536

probable potassium channel protein

  

2.00

1.93

  

sll0540

phosphate-binding protein PstS homolog

    

1.57

1.79

sll0613

Holliday junction DNA helicase RuvB

  

1.73

1.91

  

sll0621

putative c-type cytochrome biogenesis protein CcdA

    

2.22

2.00

sll0629

alternative photosystem I reaction center subunit X

  

1.94

1.72

  

sll0671

probable cation transporter

4.00

4.00

    

sll0686

probable cytochrome c-type biogenesis protein

    

3.00

3.25

sll0687

RNA polymerase ECF-type (group 3) sigma factor

  

3.00

6.00

  

sll0759

ABC transporter ATP-binding protein

    

2.13

2.09

sll0759

ABC transporter ATP-binding protein

    

2.13

2.09

sll0792

Zinc-responsive repressor ZiaR

  

3.43

3.00

  

sll0856

RNA polymerase ECF-type (group 3) sigma-E factor

    

1.89

1.61

sll1041

similar to sulfate transport ATP-binding protein CysA

  

2.63

1.63

  

sll1051

phycocyanin alpha-subunit phycocyanobilin lyase

    

8.00

13.00

sll1051

phycocyanin alpha-subunit phycocyanobilin lyase

    

8.00

13.00

sll1170

unknown protein

    

2.25

1.50

sll1223

diaphorase subunit of the bidirectional hydrogenase

1.55

2.18

    

sll1226

hydrogenase subunit of the bidirectional hydrogenase

    

1.55

1.89

sll1316

cytochrome b6-f complex iron-sulfur subunit

    

1.69

1.56

sll1330

two-component system response regulator OmpR subfamily

  

2.47

2.33

  

sll1370

mannose-1-phosphate guanylyltransferase

    

3.25

1.88

sll1423

global nitrogen regulator

  

1.57

1.55

  

sll1428

probable sodium-dependent transporter

7.00

6.00

  

5.00

2.00

sll1440

pyridoxamine 5'-phosphate oxidase

    

3.00

1.50

sll1471

phycobilisome rod-core linker polypeptide

3.90

2.77

3.56

3.07

  

sll1473

a part of phytochrome-like sensor histidine kinase gene

  

1.59

1.79

  

sll1483

periplasmic protein

    

2.00

8.40

sll1545

glutathione S-transferase

  

2.00

1.60

  

sll1612

folylpolyglutamate synthase

2.00

2.67

    

sll1679

periplasmic protease HhoA

3.35

2.42

    

sll1682

alanine dehydrogenase

    

2.91

2.27

sll1723

probable glycosyltransferase

    

1.53

4.80

sll1724

probable glycosyltransferase

    

2.11

4.89

sll1994

porphobilinogen synthase (5-aminolevulinate dehydratase)

    

1.97

1.83

sll1998

putative transposase [ISY100d: 1623697–1624643]

    

1.67

4.00

slr0018

fumarase

    

2.27

1.67

slr0051

periplasmic beta-type carbonic anhydrase

  

1.55

1.60

  

slr0070

methionyl-tRNA formyltransferase

    

1.62

1.69

slr0086

similar to DnaK protein

    

1.50

2.50

slr0089

gamma-tocopherol methyltransferase

    

4.60

3.80

slr0091

aldehyde dehydrogenase

    

7.00

11.00

slr0242

bacterioferritin comigratory protein homolog

  

1.58

1.65

  

slr0328

low molecular weight phosphotyrosine protein phosphatase

    

2.75

2.00

slr0381

lactoylglutathione lyase

    

5.14

5.00

slr0502

cobalamin synthesis protein cobW homolog

1.75

1.88

  

1.83

1.67

slr0574

cytochrome P450

    

2.34

2.41

slr0585

argininosuccinate synthetase

    

2.32

2.42

slr0618

cobyric acid synthase

  

1.69

1.56

  

slr0678

biopolymer transport ExbD like protein

    

2.19

2.26

slr0721

malic enzyme

    

1.67

2.29

slr0724

HtaR suppressor protein homolog

    

2.00

1.50

slr0741

transcriptional regulator

    

2.13

2.63

slr0758

circadian clock protein KaiC homolog

  

2.16

1.52

  

slr0819

apolipoprotein N-acyltransferase

    

2.17

1.67

slr0898

ferredoxin--nitrite reductase

  

1.65

1.53

  

slr0903

molybdopterin (MPT) converting factor, subunit 2

3.00

2.50

    

slr0940

zeta-carotene desaturase

  

1.87

2.00

2.03

1.70

slr0942

alcohol dehydrogenase [NADP+]

  

1.76

1.58

  

slr0946

arsenate reductase

    

4.50

3.50

slr0947

response regulator for energy transfer from phycobilisomes to photosystems

    

2.56

1.74

slr0949

Integral membrane protein of the ABC-type Nat permease NatD

    

14.00

7.00

slr1093

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

  

1.73

1.94

  

slr1109

similar to ankyrin

    

2.09

1.53

slr1120

type 4 prepilin-like proteins leader peptide processing enzyme

8.00

3.00

    

slr1185

cytochrome b6-f complex alternative iron-sulfur subunit

    

8.00

5.00

slr1197

SMF protein

    

1.73

1.53

slr1204

protease

  

9.52

1.96

2.45

6.65

slr1205

similar to chlorobenzene dioxygenase, ferredoxin component

    

4.00

4.00

slr1225

serine/threonine kinase

    

2.70

2.50

slr1291

NADH dehydrogenase subunit 4

    

2.44

2.44

slr1300

similar to 2-octaprenyl-6-methoxyphenol hydroxylase

    

1.59

1.59

slr1350

acyl-lipid desaturase

    

1.72

1.57

slr1379

quinol oxidase subunit I

1.56

1.70

    

slr1418

dihydroorotate dehydrogenase

    

2.50

2.63

slr1452

sulfate transport system substrate-binding protein

1.83

2.00

    

slr1596

a protein in the cytoplasmic membrane

    

2.31

2.08

slr1626

dihydroneopterin aldolase

  

1.80

3.30

  

slr1805

two-component sensor histidine kinase

    

1.60

1.98

slr1828

ferredoxin, petF-like protein

  

2.00

2.33

5.50

9.00

slr1848

histidinol dehydrogenase

    

2.15

2.06

slr1848

histidinol dehydrogenase

    

2.15

2.06

slr1853

carboxymuconolactone decarboxylase

1.80

1.70

2.71

1.59

2.56

2.55

slr1854

unknown protein

  

2.10

1.84

1.88

1.89

slr1874

D-alanine--D-alanine ligase

  

1.84

1.88

  

slr1877

2-hydroxyhepta-2,4-diene-1,7-dioate isomerase

  

2.00

1.92

  

slr1884

tryptophanyl-tRNA synthetase

    

2.09

1.72

slr1910

probable N-acetylmuramoyl-L-alanine amidase

    

1.71

2.00

slr1933

dTDP-4-dehydrorhamnose 3,5-epimerase

    

3.50

5.50

slr1938

putative translation initiation factor EIF-2b subunit 1

  

2.45

2.00

  

slr1962

probable extracellular solute-binding protein

  

1.79

1.50

  

slr1993

PHA-specific beta-ketothiolase

    

1.93

2.00

slr1994

PHA-specific acetoacetyl-CoA reductase

6.00

9.00

5.00

2.00

2.57

2.29

slr2033

membrane-associated rubredoxin

1.85

3.38

  

2.23

1.56

slr2114

perosamine synthetase

    

7.00

6.00

slr2131

RND multidrug efflux transporter

    

3.45

3.44

slr2143

L-cysteine/cystine lyase

1.67

2.56

    

smr0003

cytochrome b6-f complex subunit PetM

    

2.40

3.40

smr0009

photosystem II PsbN protein

  

1.56

1.78

  

ssl0563

photosystem I subunit VII

    

1.76

1.56

ssl0707

nitrogen regulatory protein P-II

  

2.00

1.64

  

ssl2153

probable ribose phosphate isomerase B

  

4.50

2.50

  

ssl2296

pterin-4a-carbinolamine dehydratase

  

1.75

1.86

  

ssl2542

high light-inducible polypeptide HliA, CAB/ELIP/HLIP superfamily

    

4.00

5.00

ssl3580

putative hydrogenase expression/formation protein HypC

  

3.15

2.05

  

ssr1176

putative transposase

  

1.50

1.83

  

ssr1480

putative RNA-binding protein

  

2.42

1.92

  
  1. * Induced genes encoding hypothetical proteins are provided in Additional file3: Table S3.