Spectra show the relationship between the diffraction angle (2-theta) and x-ray intensity (counts). X-ray diffraction (XRD) pattern of native, ammonia pretreated bagasse (SCAA-SB) and enzyme hydrolysed bagasse (EH-SB). Native SB showed 50.5% crystallinity index. SCAA-SB showed 65.75% crystallinity index because of high cellulose amount in substrate. EH-SB did not show major improvement in crystallinity index (67.25%).