Figure 6
From: Product inhibition of cellulases studied with 14C-labeled cellulose substrates
Inhibition of GH7 CBHs by glucose. 14C-BC (0.25 mg ml-1) was incubated with a mixture of 0.25 μM CBH and 0.025 μM EG (TrCel5A) at 35°C. The concentration of added glucose was as follows: 0 M + 0.06 μM N188BG (◊), 0 M (♦), 0.05 M (Δ), 0.125 M (+), 0.25 M (○), 0.5 M (×) or 1.0 M (□). CBH was as follows: (A) TrCel7A, (B) AtCel7A, and (C) CtCel7A. (D) Hydrolysis data in the coordinates (DGlc/DGlc=0) versus [glucose], where DGlc and DGlc=0 represent the degree of conversion of 14C-BC in the presence and absence (+N188BG series) of added glucose, respectively. (DGlc/DGlc=0) values for all hydrolysis time points are shown. CBH was TrCel7A (□), AtCel7A (Δ), and CtCel7A (×).