Figure 9

Inhibition of EGs by cellobiose. (A) ¹⁴C-amorphous cellulose (0.5 mg ml^-1) was incubated with 2.5 nM TrCel7B at 35°C. The concentration of added cellobiose was 0 mM (◊), 75 mM (□), 150 mM (×), or 225 mM (○). (B) Hydrolysis data from panel A and Additional file 1: Figure S5 in the coordinates (D_CB/D_CB=0) versus [cellobiose] where D_CB and D_CB=0 represent the degree of conversion of ¹⁴C-cellulose in the presence and absence of cellobiose, respectively. Average (D_CB/D_CB=0) values over hydrolysis time points are plotted. The solid line is from the non-linear regression according to Equation 5. TrCel7B (◊), TrCel5A (□), and TrCel12A (○).