Selecting β-glucosidases to support cellulases in cellulose saccharification

Table 2 Specificity constants of β-glucosidases for cellobiose

	k_cat(h)/K_M(h) for cellobiose (x 10⁵ M^-1 s^-1)^a
t (°C)	N188BG	TaBG3	AtBG3
25	1.66 ± 0.10	5.43 ± 0.45	3.61 ± 0.84
35	2.78 ± 0.11	7.81 ± 0.67	5.62 ± 0.77
45	3.69 ± 0.17	10.6 ± 0.60	7.99 ± 0.77
55	5.07 ± 0.44	15.5 ± 2.16	7.65 ± 0.47
65		18.4 ± 1.25	10.4 ± 0.63

^aThe k_cat(h)/K_M(h) values were calculated from the values of k_cat(h) and K_M(h) listed in Table 1.

ISSN: 2731-3654