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Table 5 Kinetic parameters of selected β-glucosidases

From: Selecting β-glucosidases to support cellulases in cellulose saccharification

Organism    kcat(s-1) KM(mM) kcat/KM(105 M-1 s-1) Kiglucose (mM) Ki/KMa Ref
  t°C pH CB pNPG CB pNPG CB pNPG on CB on pNPG   
Penicillium verruculosum    118b 650b 0.36 1.6 3.29 4.06   0.19 0.53 [34]
Phanerochaete chrysosporium 22 4 50 132 2.3 0.10 0.22 13.8   0.27 0.12 [35]
Myceliophthora thermophila 40 5 46 147 2.64 0.39 0.17 3.76   0.28 0.11 [36]
Thermoascus aurantiacus 60 4.5 284 242 0.64 0.11 4.46 21.2   0.29 0.45 [37]
Trichoderma reesei 50 4.5 22   0.54   0.41   0.29   0.54 [38]
Fomitopsis palustris 50 5 102 721 4.8 0.12 0.21 61.6   0.35 0.07 [39]
Acremonium thermophilum 55 5 666   0.87   7.65    0.50 0.57 Tc
Magnaporthe grisea 50 5    1.1     0.5   0.45 [8]
Trichoderma reesei 40 5 42 118 0.75 0.09 0.56 13.1   0.51 0.68 [40]
Chaetomium globosum 50 5 168   0.95   1.77   0.68   0.72 [21]
Trichoderma reesei 40 5 29 70.8 1.25 0.1 0.23 6.94   0.7 0.56 [41]
Penicillium verruculosum 40 5 89 160 1.2 0.44 0.74 3.64   0.93 0.78 [40]
Aspergillus fumigatus 50 5 768   1.77   4.34 0.00 1.1   0.62 [21]
Penicillium brasilianum 22 4.8 53.7b 146b 1.58 0.09 0.34 16.2 1.1 2.3 0.70 [20]
Thermoascus aurantiacus 55 5 1058   0.67   15.5    1.17 1.75 Tc
Aspergillus niger (N188) 22 4.8    0.35 0.45    1.6 1.1 4.57 [20]
Emericella nidulans 50 5 87   2.32   0.38   1.83   0.79 [21]
Aspergillus niger (N188) 50 5 558   1.15   4.85   1.94   1.69 [21]
Fusarium oxysporum 50 5 323 7.7 1.07 0.09 3.02 0.83   2.05 1.92 [42]
Penicillium brasilianum 50 5 520   2.05   2.54   2.3   1.12 [21]
Aspergillus japonicus 40 5 350 259 0.95 0.6 3.68 4.32   2.73 2.87 [40]
Aspergillus niger 25 4.5 104b 61b 2.7 1 0.38 0.61   3 1.11 [43]
Aspergillus niger (N188) 55 5 691   1.36   5.07    3.12 2.29 Tc
Trichoderma reesei 50 4.8 41 87.9 1.36 0.38 0.30 2.31   3.25 2.39 [23]
Aspergillus oryzae 50 5 363   1.78   2.04   3.26   1.83 [21]
Aspergillus niger (N188) 50 4.8 32 23.4 0.88 0.57 0.36 0.41 3.4 2.7 3.86 [23]
Aspergillus oryzae 50 5 1000 370 1.96 0.29 5.10 12.7 5 2.9 2.55 [22]
Aspergillus niger 40 4 2780 917 15.4 2.2 1.81 4.17   5.7 0.37 [44]
Aspergillus tubingensis 30 4.6 331b 140b 1 0.76 3.31 1.83   5.8 5.80 [45]
Penicillium italicum 60 4.5 2641 1746 0.41 0.11 64.4 158   8.9 21.7 [25]
Aspergillus japonicus 30 5 46b 54.5b 1.16 0.2 0.40 2.72   9.2 7.93 [46]
Neurospora crassa 50 5 423 640 2.95 2.54 1.43 2.52 10.1 6.43 3.42 [21]
Aspergillus sp 60 4.5     1.0     17 17 [47]
Periconia sp 40 5 972b 1180b 0.5 0.19 19.4 62.7   20 40.0 [48]
Baltic sea metagenome 30 6.5 11.2 22.5 2.76 0.37 0.04 0.61   30 10.8 [49]
Aspergillus niger (N188) 45 5    16.8 1.77    59.5 1.59 3.54 [24]
Streptomyces sp 50 6.5 35.6 28.4 4.1 0.15 0.09 1.89   65 15.8 [50, 51]
Torulopsis wickerhamii     362b 300 2.8   1.29   190 0.63 [52]
Thermoascus aurantiacus 40 5 0.72b 5.08b   0.2   0.25   300   [27]
Pyrococcus furiosus 95 5 454 677b 20 0.15 0.23 45.1   300 15.0 [53]
Debaromyces vanrijiae 40 5 141b 1113b 57.9 0.77 0.02 14.5   439 7.58 [54]
Aspergillus niger 40 4 4.3b 223   21.7   0.10   543   [28]
Thermoanaer. thermosacch. 70 6.4 104b 55b 7.9 0.63 0.13 0.88   600 75.9 [29]
Uncultured bacterium 40 6.5 13.2b 43b 20.4 0.39 0.01 1.11   1000 49.0 [55]
Aspergillus oryzae 50 5 253b 764b 7 0.55 0.36 13.9   1360 194 [30]
Candida peltata 50 5 54b 158b 66 2.3 0.01 0.69   1400 21.2 [31]
  1. BGs are listed in the order of increasing Ki(Glc). If Ki(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the Ki(Glc) value measured using cellobiose.
  2. aKM is for cellobiose hydrolysis. If Ki(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the Ki(Glc) value measured using cellobiose.
  3. bCalculated from the reported specific activity and molecular weight of the enzyme.
  4. cThis study.