Selecting β-glucosidases to support cellulases in cellulose saccharification

Teugjas, Hele; Väljamäe, Priit

doi:10.1186/1754-6834-6-105

Biotechnology for Biofuels and Bioproducts

Table 5 Kinetic parameters of selected β-glucosidases

From: Selecting β-glucosidases to support cellulases in cellulose saccharification

Organism			k_cat(s^-1)		K_M(mM)		k_cat/K_M(10⁵ M^-1 s^-1)		K_iglucose (mM)		K_i/K_M^a	Ref
	t°C	pH	CB	pNPG	CB	pNPG	CB	pNPG	on CB	on pNPG
Penicillium verruculosum			118^b	650^b	0.36	1.6	3.29	4.06		0.19	0.53	[34]
Phanerochaete chrysosporium	22	4	50	132	2.3	0.10	0.22	13.8		0.27	0.12	[35]
Myceliophthora thermophila	40	5	46	147	2.64	0.39	0.17	3.76		0.28	0.11	[36]
Thermoascus aurantiacus	60	4.5	284	242	0.64	0.11	4.46	21.2		0.29	0.45	[37]
Trichoderma reesei	50	4.5	22		0.54		0.41		0.29		0.54	[38]
Fomitopsis palustris	50	5	102	721	4.8	0.12	0.21	61.6		0.35	0.07	[39]
Acremonium thermophilum	55	5	666		0.87		7.65			0.50	0.57	T^c
Magnaporthe grisea	50	5			1.1				0.5		0.45	[8]
Trichoderma reesei	40	5	42	118	0.75	0.09	0.56	13.1		0.51	0.68	[40]
Chaetomium globosum	50	5	168		0.95		1.77		0.68		0.72	[21]
Trichoderma reesei	40	5	29	70.8	1.25	0.1	0.23	6.94		0.7	0.56	[41]
Penicillium verruculosum	40	5	89	160	1.2	0.44	0.74	3.64		0.93	0.78	[40]
Aspergillus fumigatus	50	5	768		1.77		4.34	0.00	1.1		0.62	[21]
Penicillium brasilianum	22	4.8	53.7^b	146^b	1.58	0.09	0.34	16.2	1.1	2.3	0.70	[20]
Thermoascus aurantiacus	55	5	1058		0.67		15.5			1.17	1.75	T^c
Aspergillus niger (N188)	22	4.8			0.35	0.45			1.6	1.1	4.57	[20]
Emericella nidulans	50	5	87		2.32		0.38		1.83		0.79	[21]
Aspergillus niger (N188)	50	5	558		1.15		4.85		1.94		1.69	[21]
Fusarium oxysporum	50	5	323	7.7	1.07	0.09	3.02	0.83		2.05	1.92	[42]
Penicillium brasilianum	50	5	520		2.05		2.54		2.3		1.12	[21]
Aspergillus japonicus	40	5	350	259	0.95	0.6	3.68	4.32		2.73	2.87	[40]
Aspergillus niger	25	4.5	104^b	61^b	2.7	1	0.38	0.61		3	1.11	[43]
Aspergillus niger (N188)	55	5	691		1.36		5.07			3.12	2.29	T^c
Trichoderma reesei	50	4.8	41	87.9	1.36	0.38	0.30	2.31		3.25	2.39	[23]
Aspergillus oryzae	50	5	363		1.78		2.04		3.26		1.83	[21]
Aspergillus niger (N188)	50	4.8	32	23.4	0.88	0.57	0.36	0.41	3.4	2.7	3.86	[23]
Aspergillus oryzae	50	5	1000	370	1.96	0.29	5.10	12.7	5	2.9	2.55	[22]
Aspergillus niger	40	4	2780	917	15.4	2.2	1.81	4.17		5.7	0.37	[44]
Aspergillus tubingensis	30	4.6	331^b	140^b	1	0.76	3.31	1.83		5.8	5.80	[45]
Penicillium italicum	60	4.5	2641	1746	0.41	0.11	64.4	158		8.9	21.7	[25]
Aspergillus japonicus	30	5	46^b	54.5^b	1.16	0.2	0.40	2.72		9.2	7.93	[46]
Neurospora crassa	50	5	423	640	2.95	2.54	1.43	2.52	10.1	6.43	3.42	[21]
Aspergillus sp	60	4.5				1.0				17	17	[47]
Periconia sp	40	5	972^b	1180^b	0.5	0.19	19.4	62.7		20	40.0	[48]
Baltic sea metagenome	30	6.5	11.2	22.5	2.76	0.37	0.04	0.61		30	10.8	[49]
Aspergillus niger (N188)	45	5			16.8	1.77			59.5	1.59	3.54	[24]
Streptomyces sp	50	6.5	35.6	28.4	4.1	0.15	0.09	1.89		65	15.8	[50, 51]
Torulopsis wickerhamii				362^b	300	2.8		1.29		190	0.63	[52]
Thermoascus aurantiacus	40	5	0.72^b	5.08^b		0.2		0.25		300		[27]
Pyrococcus furiosus	95	5	454	677^b	20	0.15	0.23	45.1		300	15.0	[53]
Debaromyces vanrijiae	40	5	141^b	1113^b	57.9	0.77	0.02	14.5		439	7.58	[54]
Aspergillus niger	40	4	4.3^b	223		21.7		0.10		543		[28]
Thermoanaer. thermosacch.	70	6.4	104^b	55^b	7.9	0.63	0.13	0.88		600	75.9	[29]
Uncultured bacterium	40	6.5	13.2^b	43^b	20.4	0.39	0.01	1.11		1000	49.0	[55]
Aspergillus oryzae	50	5	253^b	764^b	7	0.55	0.36	13.9		1360	194	[30]
Candida peltata	50	5	54^b	158^b	66	2.3	0.01	0.69		1400	21.2	[31]

BGs are listed in the order of increasing K_i(Glc). If K_i(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the K_i(Glc) value measured using cellobiose.
^aK_M is for cellobiose hydrolysis. If K_i(Glc) values measured using both pNPG and cellobiose (CB) as the substrate were available, the priority was given to the K_i(Glc) value measured using cellobiose.
^bCalculated from the reported specific activity and molecular weight of the enzyme.
^cThis study.

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