|
Metabolites
|
24 h
|
48 h
|
72 h
|
|---|
|
Cluster I
|
|
Aspartic acid
|
4
|
9
|
9
|
|
Glycerol 1-phosphate
|
7
|
9
|
6
|
|
3-phosphoglycerate
|
3
|
9
|
3
|
|
Citric acid
|
2
|
9
|
6
|
|
D-allose
|
9
|
3
|
9
|
|
Adenosine
|
5
|
9
|
8
|
|
Urea
|
1
|
7
|
3
|
|
Cluster II
|
|
Oxalic acid
|
0
|
9
|
6
|
|
Glycine
|
0
|
4
|
5
|
|
Succinic acid
|
0
|
6
|
6
|
|
L-glutamic acid (dehydrated)
|
0
|
9
|
6
|
|
Isocitric acid
|
0
|
6
|
6
|
|
Myristic acid
|
0
|
6
|
8
|
|
Sucrose
|
0
|
6
|
9
|
|
Cluster III
|
|
1,3 Propanediol
|
6
|
−6
|
−6
|
|
L-alanine
|
3
|
−3
|
−9
|
|
Itaconic acid
|
9
|
−4
|
−9
|
|
Spermidine
|
4
|
−9
|
−5
|
|
Cluster IV
|
|
Methyl palmitate
|
9
|
−5
|
6
|
|
Talose
|
9
|
−4
|
9
|
|
Benzene-1,2,4-triol
|
2
|
−3
|
6
|
|
Adrenaline
|
7
|
−6
|
9
|
|
Lauric acid
|
9
|
−8
|
7
|
|
Cluster V
|
|
L-threonine
|
7
|
9
|
0
|
|
D-malic acid
|
5
|
3
|
0
|
|
L-serine
|
6
|
5
|
0
|
|
Cluster VI
|
|
L-pyroglutamic acid
|
−3
|
−9
|
−3
|
|
Linoleic acid
|
7
|
0
|
9
|
|
L-(+) lactic acid
|
2
|
−6
|
0
|
|
Pyruvic acid
|
−6
|
0
|
9
|
|
3-hydroxypyridine
|
0
|
−5
|
9
|
|
4-hydroxypyridine
|
0
|
−3
|
8
|
|
Malonic acid
|
−5
|
−3
|
8
|
|
Caprylic acid
|
−6
|
0
|
9
|
|
Octane
|
−2
|
−5
|
8
|
|
Glycerol
|
6
|
−5
|
0
|
|
Glyceric acid
|
8
|
−9
|
0
|
|
Uracil
|
−8
|
6
|
4
|
|
Putrescine
|
−8
|
0
|
2
|
|
Tagatose
|
−5
|
0
|
9
|
|
Palmitic acid
|
0
|
−6
|
5
|
|
Methyl stearate
|
−9
|
9
|
9
|
|
D-glucose-6-phosphate
|
−3
|
8
|
9
|
|
Stearic acid
|
0
|
−8
|
5
|
|
Arachidic acid
|
−3
|
−8
|
5
|
|
D-(+) trehalose
|
−3
|
9
|
0
|
- * For each time point, three biological replicates of butanol-treated samples were compared with three biological replicates of control, generating 9 ratios. For each ratio, ratio > 1.5 was assigned as “ + 1”, ratio< − 1.5 as “-1”, and −1.5 < ratio < 1.5 as “0”. The sums of the nine ratios for each metabolite at any time point were provided.
