Table 1 Fermentation results summary of S. cerevisiae strains Y1A, Y2A, Y35 and 424A in YEP medium a
Y1A (30°C) | Y1A (35°C) | Y1A (40°C) | Y2A (30°C) | Y2A (35°C) | Y2A (40°C) | Y35 (30°C) | Y35 (35°C) | Y35 (40°C) | 424A (30°C) | 424A (35°C) | 424A (40°C) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Xylose consumption | 17.0% ± 4.5% | 7.8% ± 1.3% | 8.3% ± 0.5% | 30.4% ± 2.4% | 22.4% ± 1.4% | 9.9% ± 0.3% | 43.5% ± 0.6% | 50.9% ± 1.4% | 24.5% ± 0.4% | 99.0% ± 0.0% | 96.5% ± 0.0% | 30.3% ± 1.1% |
Maximum cell biomass (g/L) | 9.2 ± 0.5 | 8.1 ± 0.0 | 5.2 ± 0.1 | 8.7 ± 0.1 | 8.4 ± 0.1 | 5.3 ± 0.1 | 8.7 ± 0.0 | 8.3 ± 0.0 | 5.1 ± 0.2 | 8.6 ± 0.0 | 6.9 ± 0.0 | 4.2 ± 0.0 |
Yx/sb | 0.16 ± 0.01 | 0.14 ± 0.00 | 0.09 ± 0.00 | 0.15 ± 0.01 | 0.15 ± 0.00 | 0.09 ± 0.00 | 0.15 ± 0.00 | 0.14 ± 0.00 | 0.09 ± 0.00 | 0.15 ± 0.00 | 0.12 ± 0.00 | 0.07 ± 0.00 |
Xylitol (g/L) | 3.9 ± 0.8 | 2.1 ± 0.2 | 2.4 ± 0.0 | 2.7 ± 0.6 | 3.2 ± 0.2 | 1.8 ± 0.0 | 4.1 ± 0.1 | 4.2 ± 0.2 | 2.8 ± 0.2 | 1.7 ± 0.2 | 2.6 ± 0.0 | 0.9 ± 0.0 |
Glycerol (g/L) | 2.7 ± 0.3 | 2.8 ± 0.0 | 2.9 ± 0.0 | 3.5 ± 0.0 | 3.2 ± 0.0 | 3.2 ± 0.0 | 3.8 ± 0.0 | 3.8 ± 0.0 | 3.6 ± 0.0 | 4.7 ± 0.1 | 6.2 ± 0.1 | 5.3 ± 0.1 |
Specific xylose consumption rate (g/h/g cell), 18 hc | 0.012 ± 0.005 | 0.011 ± 0.007 | 0.016 ± 0.026 | 0.033 ± 0.001 | 0.022 ± 0.005 | 0.006 ± 0.003 | 0.036 ± 0.002 | 0.048 ± 0.002 | 0.044 ± 0.012 | 0.153 ± 0.000 | 0.151 ± 0.005 | 0.046 ± 0.002 |
Ethanol metabolic yield (%) | 75.6% ± 3.4% | 83.6% ± 1.1% | 83.8% ± 0.9% | 80.5% ± 0.1% | 82.3% ± 0.6% | 84.2% ± 0.4% | 79.6% ± 0.1% | 79.6% ± 0.2% | 83.2% ± 0.0% | 87.0% ± 0.8% | 84.6% ± 0.0% | 85.1% ± 0.3% |
Ethanol (g/L) | 25.4 ± 0.6 | 26.8 ± 0.2 | 26.9 ± 0.2 | 28.6 ± 0.3 | 28.2 ± 0.0 | 27.3 ± 0.1 | 29.6 ± 0.0 | 30.4 ± 0.1 | 28.5 ± 0.0 | 39.9 ± 0.4 | 38.5 ± 0.0 | 30.2 ± 0.0 |
Ethanol volumetric productivity (g/L/h)d | 0.53 ± 0.01 | 0.56 ± 0.00 | 0.56 ± 0.00 | 0.60 ± 0.01 | 0.59 ± 0.00 | 0.57 ± 0.00 | 0.62 ± 0.00 | 0.63 ± 0.00 | 0.59 ± 0.00 | 0.83 ± 0.01 | 0.80 ± 0.00 | 0.63 ± 0.00 |