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Table 1 Fermentation results summary of S. cerevisiae strains Y1A, Y2A, Y35 and 424A in YEP medium a

From: Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover

  Y1A (30°C) Y1A (35°C) Y1A (40°C) Y2A (30°C) Y2A (35°C) Y2A (40°C) Y35 (30°C) Y35 (35°C) Y35 (40°C) 424A (30°C) 424A (35°C) 424A (40°C)
Xylose consumption 17.0% ± 4.5% 7.8% ± 1.3% 8.3% ± 0.5% 30.4% ± 2.4% 22.4% ± 1.4% 9.9% ± 0.3% 43.5% ± 0.6% 50.9% ± 1.4% 24.5% ± 0.4% 99.0% ± 0.0% 96.5% ± 0.0% 30.3% ± 1.1%
Maximum cell biomass (g/L) 9.2 ± 0.5 8.1 ± 0.0 5.2 ± 0.1 8.7 ± 0.1 8.4 ± 0.1 5.3 ± 0.1 8.7 ± 0.0 8.3 ± 0.0 5.1 ± 0.2 8.6 ± 0.0 6.9 ± 0.0 4.2 ± 0.0
Yx/sb 0.16 ± 0.01 0.14 ± 0.00 0.09 ± 0.00 0.15 ± 0.01 0.15 ± 0.00 0.09 ± 0.00 0.15 ± 0.00 0.14 ± 0.00 0.09 ± 0.00 0.15 ± 0.00 0.12 ± 0.00 0.07 ± 0.00
Xylitol (g/L) 3.9 ± 0.8 2.1 ± 0.2 2.4 ± 0.0 2.7 ± 0.6 3.2 ± 0.2 1.8 ± 0.0 4.1 ± 0.1 4.2 ± 0.2 2.8 ± 0.2 1.7 ± 0.2 2.6 ± 0.0 0.9 ± 0.0
Glycerol (g/L) 2.7 ± 0.3 2.8 ± 0.0 2.9 ± 0.0 3.5 ± 0.0 3.2 ± 0.0 3.2 ± 0.0 3.8 ± 0.0 3.8 ± 0.0 3.6 ± 0.0 4.7 ± 0.1 6.2 ± 0.1 5.3 ± 0.1
Specific xylose consumption rate (g/h/g cell), 18 hc 0.012 ± 0.005 0.011 ± 0.007 0.016 ± 0.026 0.033 ± 0.001 0.022 ± 0.005 0.006 ± 0.003 0.036 ± 0.002 0.048 ± 0.002 0.044 ± 0.012 0.153 ± 0.000 0.151 ± 0.005 0.046 ± 0.002
Ethanol metabolic yield (%) 75.6% ± 3.4% 83.6% ± 1.1% 83.8% ± 0.9% 80.5% ± 0.1% 82.3% ± 0.6% 84.2% ± 0.4% 79.6% ± 0.1% 79.6% ± 0.2% 83.2% ± 0.0% 87.0% ± 0.8% 84.6% ± 0.0% 85.1% ± 0.3%
Ethanol (g/L) 25.4 ± 0.6 26.8 ± 0.2 26.9 ± 0.2 28.6 ± 0.3 28.2 ± 0.0 27.3 ± 0.1 29.6 ± 0.0 30.4 ± 0.1 28.5 ± 0.0 39.9 ± 0.4 38.5 ± 0.0 30.2 ± 0.0
Ethanol volumetric productivity (g/L/h)d 0.53 ± 0.01 0.56 ± 0.00 0.56 ± 0.00 0.60 ± 0.01 0.59 ± 0.00 0.57 ± 0.00 0.62 ± 0.00 0.63 ± 0.00 0.59 ± 0.00 0.83 ± 0.01 0.80 ± 0.00 0.63 ± 0.00
  1. aThe experiments were carried out in YEP medium with 58.2 g/L glucose and 29.8 g/L xylose, 150 rpm, 30°C. Reactions proceeded for 48 h. Standard deviations from biological duplicate experiments are indicated.
  2. bYx/s, cell biomass yield on glucose was calculated based on maximum cell biomass during glucose fermentation and consumed glucose at that time.
  3. cSpecific xylose consumption rate was calculated based on time point 18 h.
  4. dEthanol volumetric productivity was based on the final concentration of ethanol.