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Table 2 SHF fermentation results summary of S. cerevisiae strains Y35 and 424A in 6% glucan loading and 9% glucan loading ACSH at 30°C a

From: Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover

  Y35 (6%) Y35 (9%) 424A (6%) 424A (9%)
Xylose consumption 51% ± 0.0 21% ± 0.0 81% ± 0.0 39% ± 0.0
Final xylose (g/L) 14.4 ± 0.17 33.5 ± 0.16 5.53 ± 0.11 26.2 ± 0.09
Yx/sb 0.10 ± 0.00 0.07 ± 0.00 0.08 ± 0.00 0.06 ± 0.00
Xylitol (g/L) 5.0 ± 0.1 3.1 ± 0.1 0.7 ± 0.0 0.7 ± 0.0
Glycerol (g/L) 3.8 ± 0.1 5.6 ± 0.0 5.0 ± 0.0 6.0 ± 0.1
Specific xylose consumption rate (g/h/g cell), 18 hc 0.020 ± 0.001 0.016 ± 0.001 0.076 ± 0.003 0.051 ± 0.000
Sugar consumption (%)d 82.3% ± 0.2% 72.3% ± 0.1% 92.7% ± 0.1% 78.1% ± 0.1%
Ethanol metabolic yield (%) 90.0% ± 1.7% 96.6% ± 0.3% 97.6% ± 0.3% 99.7% ± 0.4%
Final Ethanol concentration (g/L) 33.3 ± 0.5 45.5 ± 0.1 40.7 ± 0.2 50.7 ± 0.1
Ethanol volumetric productivity (g/L/h), 48 he 0.61 ± 0.00 0.87 ± 0.00 0.77 ± 0.00 1.00 ± 0.01
  1. aThe experiments were carried out at 150 rpm and 30°C. Reactions proceeded for 168 h. Errors presented here were standard deviation of triplicate experiments.
  2. bYx/s, cell biomass yield on glucose was calculated based on maximum cell biomass during glucose fermentation and consumed glucose at that time.
  3. cSpecific xylose consumption rate was calculated based on time point 18 h.
  4. dSugar consumption was calculated based on available monomeric glucose and xylose for fermentation.
  5. eEthanol volumetric productivity was based on the 48 h results.