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Table 3 Summary of SSCF results on ACS using Y35 and 424A at two biomass solids loadings (6% glucan loading and 9% glucan loading) and at 30 or 35°C a

From: Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover

  Y35 (6%-30°C) Y35 (6%-35°C) 424A (6%-30°C) 424A (6%-35°C) Y35 (9%-30°C) Y35 (9%-35°C) 424A (9%-30°C) 424A (9%-35°C)
Final xylose conc. (g/L) 10.9 ± 0.8 13.3 ± 2.5 5.4 ± 0.6 13.1 ± 1.4 22.70 ± 1.35 24.69 ± 1.27 17.2 ±0.8 25.2 ± 0.6
Xylitol (g/L) 4.7 ± 0.5 4.4 ± 1.0 0.4 ± 0.0 0.6 ± 0.2 2.9 ± 1.0 4.9 ± 1.4 0.0 ±0.0 0.4 ± 0.1
Glycerol (g/L) 5.4 ± 0.4 5.5 ± 0.2 5.9 ± 0.2 6.3 ± 0.5 7.4 ± 0.4 6.8 ± 0.6 7.5 ±0.4 7.7 ± 0.4
Final Ethanol conc. (g/L) 34.9 ± 0.8 34.6 ± 1.7 40.3 ± 0.2 37.3 ± 1.0 49.5 ± 2.9 51.3 ± 0.4 54.4 ±1.3 48.8 ± 1.7
Monomeric sugar conversionb 72.5% ± 0.8% 73.7% ± 1% 71.8% ± 0.4% 73.6% ± 0.7% 68.7% ± 2.4% 71.7% ± 0.4% 69.8% ±1.5% 71.7% ± 1.7%
Sugar consumptionc 87.0% ± 1.1% 84.9% ± 3.1% 93.6% ± 0.9% 84.6% ± 1.7% 80.8% ± 1.9% 80.3% ± 0.8% 84.7% ±0.7% 74.0% ± 1.5%
Ethanol volumetric productivity (g/L/h), 48 hd 0.52 ± 0.02 0.56 ± 0.00 0.63 ±0.01 0.65 ±0.01 0.69 ±0.02 0.76 ± 0.02 0.78 ± 0.02 0.82 ± 0.02
  1. aSSCF experiments were carried out in 250 ml baffled flasks with working weight of 100 g. Shaking speed, temperature and initial pH were 180 rpm, 30°C and 5.5, respectively.
  2. bMonomeric sugar conversion was calculated based on the ethanol concentration and the remaining monomeric glucose and xylose in the fermentation broth after 168 h with the assumption of ethanol metabolic yield the same as SHF.
  3. cSugar consumption was calculated based on available monomeric glucose and xylose for fermentation.
  4. dEthanol volumetric productivity was based on the 48 h results.