Figure 2

Cell concentration and extracellular FFA concentration of targeted mutants relative to the control strain, SE02a. (A) relative cell concentration of mutants overexpressing hypothetical proteins; (B) relative extracellular FFA concentration of mutants overexpressing hypothetical proteins; (C) relative cell concentration of mutants overexpressing ROS-degrading proteins; (D) relative extracellular FFA concentration of mutants overexpressing ROS-degrading proteins; (E) relative cell concentration of knockout mutants; (F) relative extracellular FFA concentration of knockout mutants. Knockout mutants include one hypothetical protein mutant (Δ0444) and 4 putative transport proteins (Δ2175, Δ1224, Δ1464, and Δ1607). Data are averages of 3 biological replicates with error bars indicating the standard deviation of these measurements, with a few exceptions: Due to strain instabilities, mutants SB2632#1 and S1476#1 have only 2 biological replicates, and mutants SB2632#2 and S1656#2 have only 1 biological replicate. The dashed line at y = 1 indicates no change relative to the control, SE02a. An asterisk (*) following the mutant strain name in the legend indicates that difference between the control (SE02a) and mutant strain was determined to be statistically significant by ANOVA analysis (see Table S7 in Additional file 3 for calculated p-values).