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Table 2 Differentially expressed genes in S. elongatus PCC 7942 during FFA production selected for targeted mutagenesis

From: RNA-Seq analysis and targeted mutagenesis for improved free fatty acid production in an engineered cyanobacterium

Locus

Product

Average FC

Targeted Mutagenesis

Hypothetical Proteins

Synpcc7942_0444

hypothetical protein

3.27

Knockout

Synpcc7942_1561

hypothetical protein

2.67

Knockout

Synpcc7942_1023

hypothetical protein

2.15

Knockout

Synpcc7942_1476

hypothetical protein

−4.59

Overexpression

Synpcc7942_B2645

hypothetical protein

−7.35

Overexpression

Synpcc7942_1655

hypothetical protein

−2.98

Overexpression

Synpcc7942_0900

hypothetical protein

−2.92

Overexpression

Synpcc7942_B2632

hypothetical protein

−2.68

Overexpression

Synpcc7942_0122

hypothetical protein

−2.53

Overexpression

Synpcc7942_1845

hypothetical protein

−2.28

Overexpression

ROS-Degrading Proteins

Synpcc7942_1214

glutathione peroxidase

2.63

Overexpression

Synpcc7942_0801

superoxide dismutase

2.56

Overexpression

Synpcc7942_0437

glutathione peroxidase

2.54

Overexpression

Synpcc7942_1656

catalase/peroxidase HPI

−2.38

Overexpression

Potential FFA Exporters

Synpcc7942_2175

transport system substrate-binding protein

2.99

Knockout

Synpcc7942_1224

ABC-transporter membrane fusion protein

2.74

Knockout

Synpcc7942_1464

porin

2.33

Knockout

Synpcc7942_1607

porin; major outer membrane protein

2.16

Knockout

  1. Average fold change (FC) values are calculated by averaging the fold change associated with differentially expressed genes under the 6 different comparisons of high v. low FFA production.