Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

Salvachúa, Davinia; Martínez, Angel T; Tien, Ming; López-Lucendo, María F; García, Francisco; de los Ríos, Vivian; Martínez, María Jesús; Prieto, Alicia

doi:10.1186/1754-6834-6-115

Biotechnology for Biofuels and Bioproducts

Table 2 Protein identification from 2D-gel spots from I. lacteus secretomes in SSF and SmF cultures

From: Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

Spot	Day¹	SmF²	Mn³	Predicted protein function	Species⁴	Protein ID	MM (kDa)	pI	Score	UP⁵
1	7	+	+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	20.6	4
2	14	+	+	Cellulase	IL	Q9Y724	55.8	4.6	13.9	3
3	7		+	Rhamnogalacturonan-hydrolase	IL	B6E8Y7	46.7	6.9	17.4	3
4	7	+	+	Cellobiohydrolase	IL	Q75NB5	54.8	5.3	15.4	4
5	14	+	+	Cellulase	IL	Q9Y724	55.8	4.6	215.8	10
6	14	+	+	Cellulase	IL	Q9Y724	55.8	4.6	85.3	7
7	7			Cellulase	IL	Q9Y724	55.8	4.6	139.4	11
8	7	+	+	Cellobiohydrolase	IL	Q75NB5	54.8	5.3	66.7	8
9	14			Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	90.9	7
10	7	+	+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	46.0	7
11	7	+	+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	25.2	5
12	7	+	+	Endoglucanase	IL	Q5W7K4	42.2	4.9	15.4	3
13	7	+	+	Acetyl xylan esterase	PC	H2ESB9	38.9	6.5	12.9	2
14	7	+	+	Acetyl xylan esterase	PC	H2ESB9	38.9	6.5	15.5	2
15	7	+	+	Polyporopepsin	IL	P17576	35.0	4.7	20.0	5
16	7	+	+	Aspartic protease	PN	G3XKT3	42.8	5.5	70.0	2
17	14		+	Rhamnogalacturonan-hydrolase	IL	B6E8Y7	46.7	6.9	27.3	6
18	7	+	+	Acetyl xylan esterase	PC	H2ESB9	38.9	6.5	27.9	2
19	7		+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	4.1	2
20	7		+	Endo-1,4-β-xylanase A	PC	Q9HEZ1	43.5	5.4	3.1	1
21	14		+	Putative protein hypP2	MP	Q6U7U4	47.9	8.7	6.1	1
22	14		+	Putative protein hypP2	MP	Q6U7U4	47.9	8.7	6.0	1
23	14	+	+	Aspartic protease	PN	G3XKT3	42.8	5.5	3.9	1
24	14	+		Aspartic protease	PN	G3XKT3	42.8	5.5	3.7	1
25	14	+		Putative protein	PG	E3JYE0	21.2	6.3	2.7	1
26	7		+	Aspartic protease	PN	G3XKT3	42.8	5.5	3.9	1
27	7		+	Serine-type peptidase⁶	PS	Punst1 106327	59.2	4.9	53.1	1
28	7		+	Putative protein hypP2	MP	Q6U7U4	47.9	8.7	5.6	1
29	7	+	+	Cellulase	IL	Q9Y724	55.8	4.6	9.1	2
30	7	+	+	Cellobiohydrolase	IL	Q75NB5	54.8	5.3	2.7	1
31	7	+		Cellobiohydrolase	IL	Q75NB5	54.8	5.3	12.9	2
32	7	+		Cellobiohydrolase	IL	Q75NB5	54.8	5.3	6.3	2
33	7	+	+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	14.2	3
34	7		+	Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	8.5	1
35	7		+	Histone H4 (Fragment)	MP	E2LLY3	8.8	11.6	5.1	1
36	21			Cellobiohydrolase II	IL	B2ZZ24	47.2	5.3	37.1	6
37	21	+		Endoglucanase	IL	Q5W7K4	42.2	4.9	63.1	3
38	L	+		GH3/ β-glucosidase	SP	F8PMW3	78.3	4.7	63.3	4
39	L	+		Putative protein	MP	E2M3P0	13.2	4.7	3.4	1
40	L	+		Glyoxal oxidase⁶	PS	Punst1 68820	59.6	5.2	1.9	1
41	L	+		Polyporopepsin	IL	P17576	35.0	4.7	25.6	2
42	L	+		Exo-β-(1→3)-galactanase	IL	B9ZZS1	16.8	47.8	6.7	4
43	L	+		Mannose-6-phosphatase	PC	Q281W3	38.4	6.6	71.6	4

Only the hits with maximal score from Uniprot database are shown (together with two from JGI).
(1) SSF incubation time in which the spot was first detected and differential spots for SmF cultures (L). (2) and (3) + indicates presence of the spot in SmF, and SSF cultures supplemented with a manganese salt, respectively. (4) Species where the protein had been found: IL Irpex lacteus, MP Moniliophthora perniciosa, PC Phanerochaete chrysosporium, PN Pholiota nameko, PO Pleurotus ostreatus, PS Punctularia strigosozonata, and SL Serpula lacrymans. (5) Unique proteins. (6) Proteins identified only using the JGI database.

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ISSN: 2731-3654

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