Skip to main content

Advertisement

Table 2 Protein identification from 2D-gel spots from I. lacteus secretomes in SSF and SmF cultures

From: Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

Spot Day1 SmF2 Mn3 Predicted protein function Species4 Protein ID MM (kDa) pI Score UP5
1 7 + + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 20.6 4
2 14 + + Cellulase IL Q9Y724 55.8 4.6 13.9 3
3 7   + Rhamnogalacturonan-hydrolase IL B6E8Y7 46.7 6.9 17.4 3
4 7 + + Cellobiohydrolase IL Q75NB5 54.8 5.3 15.4 4
5 14 + + Cellulase IL Q9Y724 55.8 4.6 215.8 10
6 14 + + Cellulase IL Q9Y724 55.8 4.6 85.3 7
7 7    Cellulase IL Q9Y724 55.8 4.6 139.4 11
8 7 + + Cellobiohydrolase IL Q75NB5 54.8 5.3 66.7 8
9 14    Cellobiohydrolase II IL B2ZZ24 47.2 5.3 90.9 7
10 7 + + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 46.0 7
11 7 + + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 25.2 5
12 7 + + Endoglucanase IL Q5W7K4 42.2 4.9 15.4 3
13 7 + + Acetyl xylan esterase PC H2ESB9 38.9 6.5 12.9 2
14 7 + + Acetyl xylan esterase PC H2ESB9 38.9 6.5 15.5 2
15 7 + + Polyporopepsin IL P17576 35.0 4.7 20.0 5
16 7 + + Aspartic protease PN G3XKT3 42.8 5.5 70.0 2
17 14   + Rhamnogalacturonan-hydrolase IL B6E8Y7 46.7 6.9 27.3 6
18 7 + + Acetyl xylan esterase PC H2ESB9 38.9 6.5 27.9 2
19 7   + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 4.1 2
20 7   + Endo-1,4-β-xylanase A PC Q9HEZ1 43.5 5.4 3.1 1
21 14   + Putative protein hypP2 MP Q6U7U4 47.9 8.7 6.1 1
22 14   + Putative protein hypP2 MP Q6U7U4 47.9 8.7 6.0 1
23 14 + + Aspartic protease PN G3XKT3 42.8 5.5 3.9 1
24 14 +   Aspartic protease PN G3XKT3 42.8 5.5 3.7 1
25 14 +   Putative protein PG E3JYE0 21.2 6.3 2.7 1
26 7   + Aspartic protease PN G3XKT3 42.8 5.5 3.9 1
27 7   + Serine-type peptidase6 PS Punst1 106327 59.2 4.9 53.1 1
28 7   + Putative protein hypP2 MP Q6U7U4 47.9 8.7 5.6 1
29 7 + + Cellulase IL Q9Y724 55.8 4.6 9.1 2
30 7 + + Cellobiohydrolase IL Q75NB5 54.8 5.3 2.7 1
31 7 +   Cellobiohydrolase IL Q75NB5 54.8 5.3 12.9 2
32 7 +   Cellobiohydrolase IL Q75NB5 54.8 5.3 6.3 2
33 7 + + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 14.2 3
34 7   + Cellobiohydrolase II IL B2ZZ24 47.2 5.3 8.5 1
35 7   + Histone H4 (Fragment) MP E2LLY3 8.8 11.6 5.1 1
36 21    Cellobiohydrolase II IL B2ZZ24 47.2 5.3 37.1 6
37 21 +   Endoglucanase IL Q5W7K4 42.2 4.9 63.1 3
38 L +   GH3/ β-glucosidase SP F8PMW3 78.3 4.7 63.3 4
39 L +   Putative protein MP E2M3P0 13.2 4.7 3.4 1
40 L +   Glyoxal oxidase6 PS Punst1 68820 59.6 5.2 1.9 1
41 L +   Polyporopepsin IL P17576 35.0 4.7 25.6 2
42 L +   Exo-β-(1→3)-galactanase IL B9ZZS1 16.8 47.8 6.7 4
43 L +   Mannose-6-phosphatase PC Q281W3 38.4 6.6 71.6 4
  1. Only the hits with maximal score from Uniprot database are shown (together with two from JGI).
  2. (1) SSF incubation time in which the spot was first detected and differential spots for SmF cultures (L). (2) and (3) + indicates presence of the spot in SmF, and SSF cultures supplemented with a manganese salt, respectively. (4) Species where the protein had been found: IL Irpex lacteus, MP Moniliophthora perniciosa, PC Phanerochaete chrysosporium, PN Pholiota nameko, PO Pleurotus ostreatus, PS Punctularia strigosozonata, and SL Serpula lacrymans. (5) Unique proteins. (6) Proteins identified only using the JGI database.