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Table 3 Summary of the ten extracellular proteins (Top-10) identified with maximal scores from the shotgun analysis

From: Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment

EPP

Predicted protein function

Species1

ID

MM (kDa)

pI

Score

UP2

I. lacteus

Cellobiohydrolase II

IL

B2ZZ24

47.2

5.3

606.0

13

SSF

Polyporopepsin

IL

P17576

35.0

4.7

307.2

8

 

Cellulase

IL

Q9Y724

55.8

4.6

193.3

9

 

Acetyl xylan esterase

PC

H2ESB9

38.9

6.5

142.2

2

 

Melanin-decolorizing enzyme (MnP)3

Ce

B3IWB3

38.3

5.1

142.1

10

 

Peroxidase cpop21 (DyP1)4

IL

P87212

53.9

5.0

122.2

8

 

Endoglucanase

IL

Q5W7K4

42.2

4.9

118.3

3

 

Cellobiohydrolase

IL

Q75NB5

54.8

5.3

106.0

11

 

Exo-β-(1→3)-galactanase

IL

B9ZZS1

47.8

6.7

99.8

8

 

Rhamnogalacturonan-hydrolase

IL

B6E8Y7

46.7

6.9

84.5

10

I. lacteus

Polyporopepsin

IL

P17576

35.0

4.7

4867.5

13

SmF

Peroxidase cpop21 (DyP1)4

IL

P87212

53.9

5.0

684.8

13

 

Melanin-decolorizing enzyme (MnP)3

Ce

B3IWB3

38.3

5.1

368.6

11

 

Exo-β-(1→3)-galactanase

IL

B9ZZS1

47.8

6.7

202.2

11

 

Ribonuclease T2

IL

Q8LW55

41.8

5.1

137.4

4

 

Glycoside hydrolase family 3

SL

F8NLG7

89.6

5.0

130.3

1

 

Glycoside hydrolase family 3

SL

F8PMW3

78.3

4.7

111.6

2

 

Mannose-6-phosphatase

PC

Q281W3

38.4

6.6

87.5

3

 

Aspartic protease

PN

G3XKT3

42.8

5.5

63.7

3

 

Manganese peroxidase

LG

H2D7E4

38.6

4.5

56.7

3

P. chrysosporium

Manganese peroxidase 3

PC

Q1K9D0

39.8

4.6

348.7

8

SSF

Glucan 1,3-β-glucosidase

PC

Q2Z1W1

82.0

5.8

327.6

8

 

Cellulase

PC

Q7LIJ0

53.8

4.9

286.6

13

 

Copper radical oxidase

PC

Q0ZKA8

67.8

5.5

265.8

11

 

Endo-1,4-β-xylanase A

PC

Q9HEZ1

43.5

5.4

209.8

4

 

Cellobiohydrolase II (fragment)

PC

H3K419

46.3

5.1

189.6

6

 

Endo-1,4-β-xylanase C

PC

B7SIW2

42.3

4.9

151.9

6

 

Family S53 protease

PC

Q281W2

58.4

4.9

133.8

3

 

Exoglucanase 1

PC

P13860

54.8

5.5

117.1

7

 

Endo-β-glucanase

PC

C6H0M6

33.6

5.4

113.8

6

P. ostreatus

Versatile peroxidase 2 (VP2)5

PO

G8FPZ2

38.5

4.7

629.7

8

SSF

Subtilisin-like protease

PO

Q6ZYK6

93.2

5.3

330.3

11

 

Laccase

PO

Q96TR4

57.4

6.1

284.1

9

 

Putative protein

PO

D2JY75

27.8

6.6

162.8

7

 

Peptidyl-Lys metalloendopeptidase

PO

P81055

17.9

6.2

118.5

5

 

Ribonuclease T2

PO

Q75NB1

41.5

6.4

65.1

4

 

α-L-arabinofuranosidase

PO

G0TES6

68.9

8.1

52.2

3

 

Putative aspartyl-proteinase (fragment)

PO

Q96TV7

18.5

6.2

51.8

3

 

Cellulose 1,4-β-cellobiosidase

PO

A5AA53

49.3

5.6

50.6

4

 

Peptidase 1

PO

C4PFY6

38.7

8.2

42.4

2

  1. Uniprot database was used for protein identification from the secretomes of the different fungi in SSF and SmF.
  2. (1) Species where the protein had been found. Abbreviations are those used in Table 2, and: Ce Ceriporiopsis sp. and LG Lenzites gibbosa. (2) Unique proteins. (3) Nagasaki et al. [28]. (4) Salvachúa et al. [30]. (5) Camarero et al. [31].