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Table 1 Activity of the heterologously expressed enzymes against cellohexaose and 4-NPC

From: Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824

Supernatant of recombinant C. acetobutylicum strains

Substrate

Activity (units/mg total protein)

Wild-type C. aceto

Cellohexaose

NDa

Cel8A

Cellohexaose

1.1 ±0.06

Cel9B

Cellohexaose

0.02 ±0.005

thlOIDb_CipA3

Cellohexaose

ND

thlOID_Cel8A

Cellohexaose

26.9 ±4.8

thlOID_Cel9B

Cellohexaose

0.02 ±0.008

thlOID_Cel8A_CipA3

Cellohexaose

10.8 ±2.2

thlOID_Cel9B_CipA3

Cellohexaose

0.02 ±0.006

thlOID_Cel9B_Cel8A_CipA3

Cellohexaose

0.30 ±0.10

Cel9K

Cellohexaose

0.005 ±0.0001

Cel9K

4-NPCc

0.0005 ±0.0002

Wild-type C. aceto

4-NPC

ND

  1. aND: no activity detected.
  2. bthlOID: genes under control of the upstream thl and thl OID promoters.
  3. c4-NPC: 4-nitrophenyl-β-d-cellobioside.