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Table 1 Activity of the heterologously expressed enzymes against cellohexaose and 4-NPC

From: Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824

Supernatant of recombinant C. acetobutylicum strains Substrate Activity (units/mg total protein)
Wild-type C. aceto Cellohexaose NDa
Cel8A Cellohexaose 1.1 ±0.06
Cel9B Cellohexaose 0.02 ±0.005
thlOIDb_CipA3 Cellohexaose ND
thlOID_Cel8A Cellohexaose 26.9 ±4.8
thlOID_Cel9B Cellohexaose 0.02 ±0.008
thlOID_Cel8A_CipA3 Cellohexaose 10.8 ±2.2
thlOID_Cel9B_CipA3 Cellohexaose 0.02 ±0.006
thlOID_Cel9B_Cel8A_CipA3 Cellohexaose 0.30 ±0.10
Cel9K Cellohexaose 0.005 ±0.0001
Cel9K 4-NPCc 0.0005 ±0.0002
Wild-type C. aceto 4-NPC ND
  1. aND: no activity detected.
  2. bthlOID: genes under control of the upstream thl and thl OID promoters.
  3. c4-NPC: 4-nitrophenyl-β-d-cellobioside.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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