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Table 1 Determine the reference transcripts through de novo assemblies using different sets of data

From: De novo transcriptomic analysis of hydrogen production in the green alga Chlamydomonas moewusii through RNA-Seq

Contig measurements

Raw data

Trimmed data

Trimmed data with duplicates removed

N75

555

524

517

N50

1,160

1,063

1,066

N25

1,998

1,829

1,846

Minimum length (bp)

183

196

194

Maximum length (bp)

15,688

14,561

15,688

Average length (bp)

758

718

716

Contig number

39,431

33,374

34,136

Total length (Mb)

29.9

24.0

24.4

  1. Raw Data: original data generated from Illumina RNA-Seq; Trimmed Data: original data processed to trim the reads with poor quality using CLC Genomics software based on quality analysis result using FastQC software; Trimmed Data with Duplicates Removed: the trimmed reads with duplicate reads removed using CLC Genomics software.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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