Skip to main content

Advertisement

Table 1 Strains used

From: Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon

Strain Genotype Reference/construction
FTD147 ΔhyaB ΔhybC ΔhycE Skibinski et al. 2002 [43]
JW135 Δhya-Km, Δhyb-Km Menon et al. 1991 [26]
FTAB1 FTD147/ pJWPH5* This study
FTAB4 FTD147, Δ adhE, zch::Tn10, pJWPH5 P1 DC1048 (Δ adhE::Tn10),
FTAB5 FTD147 Δ arcA ::Tn10, pJWPH5 P1 QC2575 (Δ arcA::Tn10),
FTDPH10 FTD147/pJWPH5 adhE, ldhA P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10)
DG2 FTD147/pJWPH5 adhE, ldhA, arcA P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), QC2575 (Δ arcA::Tn10)
FTJWDC3 FTD147/pJWPH5 adhE, mdh P1 DC1048 (Δ adhE::Tn10), JW3205-1 (Δ mdh::Tn5)
FTGH2 FTD147/pJWPH5 adhE, ldhA, mdh P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), JW3205-1 (Δ mdh::Tn5)
DJ1 JW135/pJWPH5 arcA P1 QC2575 (Δ arcA::Tn10)
JWGH1 JW135/pJWPH5 adhE, ldhA, arcA P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), QC2575 (Δ arcA::Tn10)
  1. *Plasmid containing the Ralstonia eutropha SH operon under the control of the trc promoter in pTrc99A.