Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon

Table 1 Strains used

Strain	Genotype	Reference/construction
FTD147	ΔhyaB ΔhybC ΔhycE	Skibinski et al. 2002 [43]
JW135	Δhya-Km, Δhyb-Km	Menon et al. 1991 [26]
FTAB1	FTD147/ pJWPH5*	This study
FTAB4	FTD147, Δ adhE, zch::Tn10, pJWPH5	P1 DC1048 (Δ adhE::Tn10),
FTAB5	FTD147 Δ arcA ::Tn10, pJWPH5	P1 QC2575 (Δ arcA::Tn10),
FTDPH10	FTD147/pJWPH5 adhE, ldhA	P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10)
DG2	FTD147/pJWPH5 adhE, ldhA, arcA	P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), QC2575 (Δ arcA::Tn10)
FTJWDC3	FTD147/pJWPH5 adhE, mdh	P1 DC1048 (Δ adhE::Tn10), JW3205-1 (Δ mdh::Tn5)
FTGH2	FTD147/pJWPH5 adhE, ldhA, mdh	P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), JW3205-1 (Δ mdh::Tn5)
DJ1	JW135/pJWPH5 arcA	P1 QC2575 (Δ arcA::Tn10)
JWGH1	JW135/pJWPH5 adhE, ldhA, arcA	P1 DC1048 (Δ adhE::Tn10), SE1752 (Δ ldhA::Tn10), QC2575 (Δ arcA::Tn10)

^*Plasmid containing the Ralstonia eutropha SH operon under the control of the trc promoter in pTrc99A.