Figure 5

Time course of fed-batch fermentation of 2,3-BD. Biomass (▼), 2,3-BD (●), glucose (■), AC (▲), acetic acid (Δ), lactic acid (□), ethanol (▽) and formic acid (○). The fermentation was carried out at 50°C in a 5-L bioreactor with pH control by automatic addition of 6 M acetic acid and NaOH, stirring at 400 rpm in the first 10 h and then decreased to 200 rpm, and airflow at 1.0 vvm.