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Figure 7 | Biotechnology for Biofuels

Figure 7

From: A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical

Figure 7

SDS-PAGE of the purified enzymes and GC analysis of the reaction products from AC. A, SDS-PAGE analysis of the purified meso-BDH: M, marker; line 1, purified meso-BDH; line 2, crude extract of E. coli BL21 (DE3) (pETDuet-bdh) induced by IPTG. B, GC analysis of the meso-BDH catalyzed products of AC with NADH as a cofactor. C, SDS-PAGE analysis of the purified GDH: M, marker; line 1, purified GDH; line 2, crude extract of E. coli BL21 (DE3) (pETDuet-gdh) induced by IPTG. D, GC analysis of the GDH catalyzed products of AC with NADH as a cofactor. * Isoamyl alcohol was used as the internal standard in B and D.

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