Figure 7

SDS-PAGE of the purified enzymes and GC analysis of the reaction products from AC. A, SDS-PAGE analysis of the purified meso-BDH: M, marker; line 1, purified meso-BDH; line 2, crude extract of E. coli BL21 (DE3) (pETDuet-bdh) induced by IPTG. B, GC analysis of the meso-BDH catalyzed products of AC with NADH as a cofactor. C, SDS-PAGE analysis of the purified GDH: M, marker; line 1, purified GDH; line 2, crude extract of E. coli BL21 (DE3) (pETDuet-gdh) induced by IPTG. D, GC analysis of the GDH catalyzed products of AC with NADH as a cofactor. * Isoamyl alcohol was used as the internal standard in B and D.