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Figure 1 | Biotechnology for Biofuels

Figure 1

From: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

Figure 1

Schematic diagram of the knockout strategy for the pta and ack genes. The pta-ack locus on the T. aotearoense SCUT 27 chromosome, the pBluescript II SK(+) derived knock out plasmid pPuKAd used to disrupt the pta-ack gene locus and the predicted pta-ack gene locus after double cross over integration are shown. The endonucleolytic cleavage sites used in the pPuKAd construction are indicated. The location of the probe and the expected sizes of the fragments detected by southern blot analysis of the genomic DNA digested with Pst I are shown.

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