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Table 1 Batch fermentation comparison of SCUT27 and LA1002 of T. aotearoense a

From: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

  Glucose Xylose
SCUT27 LA1002 Foldg SCUT27 LA1002 Fold
Carbon recoveryb 106.02 93.8 - 95.26 99 -
Final pH 3.76 3.77 - 3.81 3.65 -
DCW (g/L) 0.93 0.65 0.70 0.79 0.55 0.69
Consumed sugar (g/L) 7.08 4.83 0.68 6.43 4.23 0.66
CLAc (g/L) 2.36 2.85 1.21 1.82 2.43 1.34
YLAd (g/g) 0.33 0.59 1.79 0.28 0.58 2.07
PLAe (g/L/h) 0.19 0.29 1.53 0.20 0.22 1.10
SPLAf (g/g cells) 2.54 4.39 1.73 2.29 4.44 1.94
CEtOH (g/L) 1.22 0.59 0.49 0.98 0.46 0.47
CAc (g/L) 0.88 0.00 - 0.73 0.00 -
H2 (mL/L) 786.76 261.34 0.33 605.93 161.43 0.27
  1. a Batch fermentation was performed in 125 mL serum bottles containing 50 mL of modified MTC medium, and cultivated with initial pH 6.0 at 55°C for 24 h. The experiments were done on three independent repeats and the standard deviation were not showed in this table for the purposes of simplicity.
  2. b Carbon recovery accounts for the average percentage of carbon recovered in all products and biomass at all time points.
  3. c Lactic acid concentration (g/L).
  4. d Yield of lactic acid produced (g) to consumed sugar (g).
  5. e Lactic acid productivity, calculated as the ratio of lactic acid concentration (g/L) to the fermentation time during the exponential growth.
  6. f Lactic acid specific productivity, in gram lactic acid per gram of cells per hour, were calculated as the ratio of lactic acid concentration to dry cell weight (DCW) during the exponential phase.
  7. g The fold values were calculated by dividing the data of LA1002 by those of SCUT27.