Skip to main content

Table 2 Fermentation parameters in batch cultivation by LA1002 a

From: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

Substrate

Sterilized

Carbon recoveryb

μ max

DCW

Timec

C LA

Y LA

P LA

C EtOH

CLA/CEtOH

(%)

(h-1)

(g/L)

(h)

(g/L)

(g/g)

(g/L/h)

(g/L)

50 g/L glucose

Yes

107 ± 6

0.37

1.69

36

47.17

0.93

2.60

3.66

12.89

50 g/L xylose

Yes

100 ± 1

0.23

1.78

84

39.72

0.79

0.65

4.63

8.58

25 g/L glucose, 25 g/L xylose

Yes

101 ± 2

0.37

2.10

48

43.56

0.86

1.85

4.12

10.57

25 g/L glucose, 25 g/L xylose

No

98 ± 7

0.32

2.16

60

44.89

0.89

1.26

4.01

11.19

  1. a Batch fermentation was performed in 5 L fermentor containing 3 L medium and cultivated at 55°C controlling pH as 6.5 with 5 M of sodium hydroxide. Data represent the average results from three independent experiments.
  2. b Carbon recovery accounts for the average of percentage of carbon recovered in all products and biomass at all the time points.
  3. c The specified time that strain reached the maximum lactic acid concentration.