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Table 2 Fermentation parameters in batch cultivation by LA1002 a

From: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity

Substrate Sterilized Carbon recoveryb μ max DCW Timec C LA Y LA P LA C EtOH CLA/CEtOH
(%) (h-1) (g/L) (h) (g/L) (g/g) (g/L/h) (g/L)
50 g/L glucose Yes 107 ± 6 0.37 1.69 36 47.17 0.93 2.60 3.66 12.89
50 g/L xylose Yes 100 ± 1 0.23 1.78 84 39.72 0.79 0.65 4.63 8.58
25 g/L glucose, 25 g/L xylose Yes 101 ± 2 0.37 2.10 48 43.56 0.86 1.85 4.12 10.57
25 g/L glucose, 25 g/L xylose No 98 ± 7 0.32 2.16 60 44.89 0.89 1.26 4.01 11.19
  1. a Batch fermentation was performed in 5 L fermentor containing 3 L medium and cultivated at 55°C controlling pH as 6.5 with 5 M of sodium hydroxide. Data represent the average results from three independent experiments.
  2. b Carbon recovery accounts for the average of percentage of carbon recovered in all products and biomass at all the time points.
  3. c The specified time that strain reached the maximum lactic acid concentration.