Efficient production of l -lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity

Table 2 Fermentation parameters in batch cultivation by LA1002 ^a

Substrate	Sterilized	Carbon recovery^b	μ _max	DCW	Time^c	C _LA	Y _LA	P _LA	C _EtOH	C_LA/C_EtOH
Substrate	Sterilized	(%)	(h^-1)	(g/L)	(h)	(g/L)	(g/g)	(g/L/h)	(g/L)	C_LA/C_EtOH
50 g/L glucose	Yes	107 ± 6	0.37	1.69	36	47.17	0.93	2.60	3.66	12.89
50 g/L xylose	Yes	100 ± 1	0.23	1.78	84	39.72	0.79	0.65	4.63	8.58
25 g/L glucose, 25 g/L xylose	Yes	101 ± 2	0.37	2.10	48	43.56	0.86	1.85	4.12	10.57
25 g/L glucose, 25 g/L xylose	No	98 ± 7	0.32	2.16	60	44.89	0.89	1.26	4.01	11.19

^a Batch fermentation was performed in 5 L fermentor containing 3 L medium and cultivated at 55°C controlling pH as 6.5 with 5 M of sodium hydroxide. Data represent the average results from three independent experiments.
^b Carbon recovery accounts for the average of percentage of carbon recovered in all products and biomass at all the time points.
^c The specified time that strain reached the maximum lactic acid concentration.