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Table 1 Physiological analysis of S. cerevisiae IMU033 expressing PRK and Rubisco and the isogenic reference strain IMU032 in anaerobic chemostat cultures, grown at a dilution rate of 0.05 h -1 on a synthetic medium (pH 5) supplemented with 12.5 g l -1 glucose and 12.5 g l -1 galactose as carbon sources #

From: Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast

  IMU032 IMU033
  (Reference strain) (Expressing PRK and Rubisco)
CO2 in inlet gas (%) 0 10 0 10
CO2 in outlet gas (%) 0.89 ± 0.03 10.8 ± 0.0 1.02 ± 0.00 10.8 ± 0.1
Phosphoribulokinase (μmol mg protein-1 min-1) 0.58 ± 0.09 0.51 ± 0.12 14.4 ± 1.5 15.2 ± 1.0
Rubisco (nmol mg protein-1 min-1) < 0.2* < 0.2 4.59 ± 0.30 2.67 ± 0.28
Biomass yield on sugar (g g-1) 0.083 ± 0.000a 0.084 ± 0.000b 0.093 ± 0.001a 0.095 ± 0.000b
Ethanol yield on sugar (mol mol-1) 1.56 ± 0.03c 1.56 ± 0.02d 1.73 ± 0.02c 1.73 ± 0.01d
Glycerol yield on sugar (mol mol-1) 0.14 ± 0.00e 0.12 ± 0.00f 0.04 ± 0.00e, g 0.01 ± 0.00f, g
  1. #Results are represented as average ± mean deviations of data from independent duplicate chemostat experiments. Data pairs labelled with the same subscripts (a,a, b,b, etc.) are considered statistically different in a standard t-test (p <0.02).
  2. *Detection limit of enzyme activity assay.