Figure 3

Avicelase activity of the designer minicellulosome and its proximity synergy. Activities of the minicellulosome (uppermost curve), its individual catalytic protein subunits (lowermost two curves), and a simple mixture of the two catalytic subunits (second curve from top) were analyzed. As in Figure 2, all catalytic protein subunits (with the exception of the lowermost curve, which is presented as a dashed line) were previously complexed with a CBM-bearing binding adaptor, either a single-cohesin scaffoldin or, in the case of the minicellulosome, a two-cohesin scaffoldin with each of the two cohesins specific for the dockerin module borne by one of the catalytic subunits. The lowermost (dashed) line represents Avicel conversion by a “bare-dockerin” version of the modified Cel48S subunit, i.e., one not provided with the C. cellulolyticum-cohesin/CBM3a binding adaptor matching its dockerin. Assay conditions as in Figure 2. For key to icons, please see Figure 1 and Table 1.