Figure 4
From: Improving activity of minicellulosomes by integration of intra- and intermolecular synergies
Assembly of the minicellulosome and its verification by native PAGE. Lane 1, chimeric scaffoldin (CBM3a-Coh-Coh); 2, modified Cel48S (CBM4-Ig-GH48-Doc); 3, artificial multicatalytic cellulase (CBM4-Ig-GH9-X11-X12-GH8-Doc); 4, complex formed by mixing chimeric scaffoldin and modified Cel48S; 5, completed minicellulosome formed by mixing artificial multicatalytic cellulase with complex shown in Lane 4. For key to icons, please see Figure 1 and Table 1.