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Table 1 Expressed proteins prepared in this study

From: Improving activity of minicellulosomes by integration of intra- and intermolecular synergies

Protein

Module architecture

Gene source

Plasmid

Tag

Modified Cel48S-T

CBM4-Ig-GH48-DocA

C. thermocellum

pET28

C-terminal His-tag

Modified Cel48S-C

CBM4-Ig-GH48-DocB

C. thermocellum C. cellulolyticum

pET28

C-terminal His-tag

Cel8A

GH8-Doc

C. thermocellum

pET28

C-terminal His-tag

Truncated CbhA

CBM4-Ig-GH9-X11-X12-DocC

C. thermocellum

pET22

C-terminal His-tag

Bi-functional cellulase

CBM4-Ig-GH9-X11-X12-GH8-DocD

C. thermocellum

pET22

C-terminal His-tag

Truncated CipA

Coh-CBM3aE

C. thermocellum

pET28

C-terminal His-tag

Chimeric scaffoldin

Coh-CBM3aF

C. thermocellum C. cellulolyticum

pET28

C-terminal His-tag

Chimeric scaffoldin

CBM3a-Coh-CohG

C. thermocellum C. cellulolyticum

pET28

C-terminal His-tag

  1. Note: GH, glycoside hydrolase family; CBM, carbohydrate binding module; Coh, cohesin; Doc, dockerin; Ig, immunoglobulin-like fold. AThe “CBM4-Ig” sequence segment is derived from C. thermocellum CbhA, and “GH48-Doc” from Cel48S. B“CBM4-Ig” and –“GH48” are derived from C. thermocellum CbhA and Cel48S respectively, but here, “Doc” is from C. cellulolyticum Cel48F. CEffectively the sequence of CbhA with the CBM3b excised. D“CBM4-Ig-GH9-X11-X12” is the N-terminal portion of CbhA, and “GH8-Doc” is the sequence of C. thermocellum Cel8A. EExcised internal segment of C. thermocellum CipA (Coh2 and CBM3a). F “Coh” is the Coh1 of C. cellulolyticum CipC and “CBM3a” is from C. thermocellum CipA. G“CBM3a” is from C. thermocellum CipA; the first “Coh” is the Coh1 of C. cellulolyticum CipC, and the second “Coh” is the Coh4 of C. thermocellum CipA.