Improving activity of minicellulosomes by integration of intra- and intermolecular synergies

Table 2 Primers used for this study

Primer^A	Nucleotide sequence^B	Gene cloning or construction
F-V8-NdeI	TCCGTGCATATG TTAGAAGATAATTCTTCGACT	CbhA
R-V8D-XhoI	CTGTACCTCGAG ATCCCGTGCCTGTTTTACAA	CbhA
F-NcoI-cels1	CTGCATCCATGG GTCCTACAAAGGCACCTAC	Cel48S
R-XhoI-cels1	ATCAGTTTTGCTCGAG GTTCTTGTACGGCAATGTAT	Cel48S
F-V7-NcoI	CTGTGTCCATGG CAGGTGTGCCTTTTAACACA	Cel8A
R-V7-XhoI	CCCATTCTCGAG ATAAGGTAGGTGGGGTATGC	Cel8A
F-V7-XhoI	ACTGTGCTCGAG GCAGGTGTGCCTTTTAA	Cel8A

Note: ^AOther genes listed Table 1 were synthesized by GenScript, and their primers are not listed. ^BResidues underlined are the designated restriction enzyme cleavage sites.

ISSN: 2731-3654