Chromosomal distribution of jatropha copia -type retrotransposons. Mitotic metaphase spread of J. curcas (2n = 22) after fluorescence in situ hybridization with RT element had high, moderate and low copy number as a probe. The chromosomes were counterstained light blue with DAPI. Green and red signal represent RT sequences labeled with DIG and biotin, respectively. (a) RT sequence belongs to family Jc1 (high copy), (b) Jc5 (high copy), (c) Jc3 (moderate copy) and (d) Jc8 (low copy) were used as probes. (e) Double fluorescence in situ hybridization with the 5S rRNA gene sequence and the RT sequence of Jc5. Bars= 3 μm.