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Table 2 Strains used in this study

From: Improvement of n-butanol tolerance in Escherichia coliby membrane-targeted tilapia metallothionein

Strains

Genotype and description

Reference or source

E. coli BL21

E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)

Invitrogen

PET30a

E. coli BL21/ PET30a , T7 promoter, f1 origin, Kmr

Novagen, Inc.

HMT

E. coli BL21/PET30a;hmt (human metallothionein)

[26]

MMT

E. coli BL21/PET30a;mmt (mouse metallothionein)

[26]

TMT

E. coli BL21/PET30a;tmt (tilapia metallothionein)

[26]

OmpC

E. coli BL21/PET30a;ompC (E. coli outer membrane protein C)

[26]

OmpC-HMT

E. coli BL21/PET30a;ompC-hmt

[26]

OmpC-MMT

E. coli BL21/PET30a;ompC-mmt

[26]

OmpC-TMT

E. coli BL21/PET30a;ompC-tmt

[26]