Figure 1

MCR model describing sample contamination, washing and tape support for samples treated in 96-well filter plates. Samples in the model included Scotch® tape alone, extracted spruce that had not been exposed to the filter plate or to buffer (“no plate, no buffer”), and extracted spruce incubated in buffer on the 96-well filter plate and then washed on-plate with either continuous or intermittent vacuum (“plate, buffer, cont wash” or “plate, buffer, int wash”). Scores plots (B,C,F,G,J) show how well the positive ion ToF-SIMS spectra match each pure component spectrum describing buffer salts (A), PDMS contamination (D), tape (E,I) and spruce (H).