From: Towards practical time-of-flight secondary ion mass spectrometry lignocellulolytic enzyme assays
Original assays in 3 mL glass vials[1] | Assays using 96-well filter plates |
---|---|
15–20 mg of wood per vial | 4–5 mg of wood per well |
1–2 mL of solution per vial | 250 μL of solution per well |
Manual sample washing was tedious, requiring settling and pipetting times exceeding 2 h for 96 samples | Whole plate is washed in parallel using a vacuum manifold (20–30 min, robotic or manual for 96 wells) |
Wood fibers were individually pressed into 13-mm pellets and mounted for ToF-SIMS, a 4 h process for 96 samples | Wood fibers are transferred to adhesive tape for ToF-SIMS analysis, a 10 minute process for all 96 wells |