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Table 1 Time and material savings using 96-well filter plates

From: Towards practical time-of-flight secondary ion mass spectrometry lignocellulolytic enzyme assays

Original assays in 3 mL glass vials[1]

Assays using 96-well filter plates

15–20 mg of wood per vial

4–5 mg of wood per well

1–2 mL of solution per vial

250 μL of solution per well

Manual sample washing was tedious, requiring settling and pipetting times exceeding 2 h for 96 samples

Whole plate is washed in parallel using a vacuum manifold (20–30 min, robotic or manual for 96 wells)

Wood fibers were individually pressed into 13-mm pellets and mounted for ToF-SIMS, a 4 h process for 96 samples

Wood fibers are transferred to adhesive tape for ToF-SIMS analysis, a 10 minute process for all 96 wells