Figure 5

Identification of the genes derived from S. padanus involved in improving xylose metabolism in R. opacus PD630. (a) Physical map of the 3603-bp insert on pXsp8. The numbers and arrows in the ORF boxes indicate those of the amino acid residue and the locations of ORFs, respectively. (b-d) Pairwise identities of amino acid sequences using the CLUSTALW program. (b) The ORF 1 (388 aa) and xylose isomerases of S. hygroscopicus accession no. AEY87893 (388 aa), S. avermitilis accession no. NP_828358 (388 aa), S. chartreusis accession no. ZP_09957234 (388 aa) and S. coelicolor accession no. NP_625460 (387 aa). (c) Xylulose kinase domain (XKD, 410 aa) of the ORF 2 and xylulose kinases of S. hygroscopicus accession no. AEY87894 (481 aa), S. avermitilis accession no. NP_828357 (481 aa), S. chartreusis accession no. ZP_09957235 (481 aa) and S. coelicolor accession no. NP_733525 (481 aa). (d) Cellulose-binding domain (CBD, 235 aa) of the ORF 2 and cellulose-binding proteins of S. hygroscopicus accession no. AEY91743 (311 aa), S. avermitilis accession no. NP_824035 (312 aa), S. chartreusis accession no. ZP_09956458 (311 aa) and S. coelicolor accession no. NP_629535 (310 aa).