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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Strong cellulase inhibitors from the hydrothermal pretreatment of wheat straw

Figure 2

Separation of inhibitory compounds from the LF. The LF was fractionated using size exclusion chromatography. Fractions were analyzed for the reducing groups (white columns) and the inhibitory power against Tr Cel7A on MUL (gray columns) using 100 times diluted fractions. HPLC chromatograms of the selected fractions are shown in the insets. Fractions with elution volumes between 155.0 and 172.5 ml (Mw range of 1.0 – 3.0 kDa, indicated with red arrows) were pooled and purified using HPLC.

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